Rabbit Anti-Ring1A Polyclonal Antibody#abs131143

abs131143-50ug

1-2 Weeks

201

abs131143-100ug

1-2 Weeks

301

Description

One of the main E3 ubiquitin-protein ligases responsible for the monoubiquitination of histone H2A at the 'Lys-119' residue, which is crucial for regulating gene expression through the histone code. This modification, known as H2A 'Lys-119' ubiquitination, serves as a specific marker for epigenetic transcriptional repression and plays a key role in X chromosome inactivation in female mammals. It is an integral component of the Polycomb group (PcG) multiprotein PRC1 complex, which is required for maintaining a repressive state in many genes, including Hox genes, throughout development.

Other names

The protein known as Ring1A, or Really Interesting New Gene 1 protein, is a crucial component of the Polycomb complex, as well as an E3 ubiquitin-protein ligase. As a RING finger protein, Ring1A functions as a transcriptional repressor, and is involved in the regulation of important cellular processes. Its primary role is in the binding and modification of specific target proteins, which ultimately leads to their degradation. With multiple names and a complex set of functions, Ring1A's importance in the body cannot be overstated.

Source

Rabbit

Specificity

The total Ring1A protein's endogenous level is detected by the antibody. Generating highly similar content by rearranging the above content, while ensuring it is based on the original information provided.

Predictive reaction species

Pig;Sheep;Dog;Horse;

Application

The recommended dilutions for this antibody include WB at 1:500-1:1000, IF/ICC at 1:100-1:500, and ELISA(peptide) at 1:20000-1:40000. To create similar content, the same information can be conveyed by rephrasing the original text. It is suggested to use this antibody at a dilution of 1:500-1:1000 for Western blotting, 1:100-1:500 for immunofluorescence and immunocytochemistry, and 1:20000-1:40000 for ELISA with peptide.

Ring1Aaa.35~39（I-A-V-S-P）。，。

Properties

1mg/ml

Purification

The SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography in order to purify the antiserum.

Clonality

Polyclonal Antibody

To maintain its quality, it is advised to store the product at -20 °C for a period of one year. To prevent any potential damage, it is important to avoid subjecting it to repeated freeze/thaw cycles.

Storage buffer

This is a description of Rabbit IgG in a solution consisting of phosphate buffered saline at pH 7.4, 150 mM NaCl, and 0.02% sodium azide with 50% glycerol. The solution should be stored at -20 °C, and it will remain stable for up to 12 months from the date of receipt.

Target

Background

 Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.

Celluar localization

Q06587