Mouse IL-9 ELISA Kit

SKU-Pack Size	Availability	Price
abs552008-96T	4 weeks	$450.00

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Testing Principle：

This particular kit utilizes the double antibody sandwich ELISA technique. The conjugate plate provided in the kit already has the specific anti-human CXCL13 Capture Antibody attached to it, demonstrating a high affinity for the target. To conduct the assay, the standard substance, as well as the sample being tested, are sequentially added to the plate, followed by the addition of the biotin-labeled detection antibody. After thorough mixing, the plate is incubated at room temperature for 2 hours, allowing the CXCL13 in the sample to bind to both the solid-phase and detection antibodies.
To remove any unbound components, the plate is thoroughly washed. Next, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is introduced to the plate. After another round of washing, the plate is treated with TMB color substrate and incubated in a dark environment at room temperature for color development. The intensity of the resulting color reaction is directly proportional to the concentration of CXCL13 present in the sample.
To halt the reaction, a termination solution is added to the plate. The absorbance of the developed color is then measured using a microplate analyzer at a detection wavelength of 450 nm (with a correction wavelength range of 570-630 nm). This measurement allows for the quantification of CXCL13 concentration in the sample.

Detection type：Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity：100 μl

Kit composition：The package includes the following items: a pre-coated 96-well plate, Standards, Standard Diluent, Test Buffer, One copy of CXCL13 test antibody, TMB Color Substrate, Washing Solution, Stop Solution, SA-HRP, Seal Plate Film, and comprehensive Instructions. These components have been carefully arranged to provide users with a complete and convenient CXCL13 test kit.

Sensitivity：3.59 pg/mL

Detection range： 125-8,000 pg/mL

Recovery rate： 95-117%

Storage：2-8℃

The standard curve：

 

Background：

IL-9, also known as Interleukin 9, is a glycosylated protein with a molecular weight of 14kDa. It consists of 10 cysteine residues that play a crucial role in forming disulfide bonds. Produced primarily by T cells, particularly CD4+ helper cells, IL-9 acts as a cytokine that regulates various hematopoietic cells. Its main functions include promoting cell proliferation and inhibiting cell apoptosis.
IL-9 functions by binding to the IL-9 receptor, which then activates different signal transduction and activation (STAT) proteins. This activation leads to the participation of IL-9 in a wide range of biological processes. Interestingly, studies have identified the gene encoding IL-9 as a potential candidate gene for asthma.
Further genetic research conducted on mouse models of asthma suggests that IL-9 plays a crucial role in the development of bronchial hyperresponsiveness. The presence of an IL-9-mediated autocrine loop supports the involvement of this cytokine in certain malignancies, including Hodgkin's disease. Expression of IL-9 has been observed in Lee-Scherer cells, Hodgkin's lymphoma cells, and some large aplastic lymphoma cells. However, it is notably absent in non-Hodgkin's lymphoma and peripheral T-cell lymphoma.
In essence, IL-9 stands as a pivotal cytokine with significant implications in various physiological and pathological processes. Further research on IL-9 and its associated signaling pathways may provide valuable insights into the development and treatment of asthma and certain malignancies.

 

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