Human IL-4 High Sensitivity ELISA Kit

SKU-Pack Size	Availability	Price
abs551803-96T	4 weeks	$580.00

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Testing Principle：

The double antibody sandwich ELISA method is employed in this kit. The conjugate plate already contains specific anti-human CXCL13 Capture Antibody with a high affinity. The process begins with the addition of the standard substance, the sample to be tested, and the biotin-labeled detection antibody into the HRP-plate well, one after the other. Thorough oscillation and mixing ensure the interaction of CXCL13 in the sample with the solid phase antibody and detection antibody during a 2-hour incubation at room temperature.
To eliminate any unbound ingredients, extensive washing is performed. Subsequently, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is introduced. Following another round of washing, a TMB color substrate is added and allowed to incubate in darkness at room temperature, promoting color development. The intensity of the color reaction directly corresponds to the concentration of CXCL13 present in the sample.
To stop the reaction, a termination solution is added. Utilizing a microplate analyzer, the absorbance value is measured at a detection wavelength of 450 nm (with correction wavelength set between 570-630 nm). This ensures accurate quantification of the CXCL13 concentration in the sample.

Detection type：Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity：100 μl

Kit composition：Included in the package is a pre-coated 96-well plate, along with Standards, Test Antibody, Standard Diluent, Test Buffer, TMB Color Substrate, Washing Solution, Stop Solution, SA-HRP, Seal Plate Film and easy-to-follow instructions for use. This kit is designed to provide highly accurate results for CXCL13 testing in a convenient and reliable way. The standards provided help to ensure consistency and accuracy in the results obtained, while the pre-coated plate simplifies the testing process and saves time. With all the necessary reagents provided in this kit, you can expect reliable and consistent results every time.

Sensitivity：0.03 pg/mL

Detection range： 0.25-16 pg/mL

Recovery rate： 94-105%

Storage：2-8℃

The standard curve：

 

Background：

IL-4, a monomer cytokine with a molecular weight ranging from 18-20kDa, plays a pivotal role in driving the differentiation of Th0 cells into Th2 cells. The activation of Th2 cells is primarily induced by IL-4, which triggers positive feedback and further secretion of this cytokine. Although the exact type of cell that secretes IL-4 to promote Th0 cell differentiation remains undefined, research reveals that basophils may play a crucial role in this process. IL-4 and IL-13 have an intimate relationship and share similar functions in various physiological processes.
Rhabdomyosarcoma, a type of cancer, is influenced by interleukin 4 (IL-4) as it stimulates cellular division, induces dedifferentiation, and contributes to the spread of the disease. In addition, IL-4, along with other T helper 2 (Th2) cytokines, is linked to inflammatory responses in the airways of individuals with allergic asthma.

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