Tubulin Beta Antibody#abs131994
Please note that the price mentioned above is only for your reference. If you require more detailed pricing information, kindly get in touch with our sales representative Vecent. Western blot analysis of Tubulin beta expression in Mouse Brain, whole cell lysates,The lane on the left is treated...
Description
Catalog-specification | Delivery time | USD price |
abs131994-50ug | 1-2 Weeks | 201 |
abs131994-100ug | 1-2 Weeks | 301 |
Please note that the price mentioned above is only for your reference. If you require more detailed pricing information, kindly get in touch with our sales representative Vecent.
Overview | |
Description | TUBB3 Tubulin, a vital component of microtubules, exhibits a strong affinity for GTP, binding both the exchangeable site on the beta chain and the non-exchangeable site on the alpha-chain. Its crucial involvement in maintaining accurate axon guidance cannot be overstated. The role TUBB3 plays in sustaining proper axon guidance is of utmost significance, ensuring the proper functioning and preservation of neuronal pathways. |
| Source | Rabbit |
| Specificity | The Tubulin beta Antibody is capable of detecting the total endogenous levels of Tubulin beta. To create similar content, the original text can be paraphrased by stating that the antibody is capable of identifying the complete amount of Tubulin beta present in any given sample. This offers a reliable means of detecting the endogenous levels of Tubulin beta and assessing its biological activity. |
| Species Reactivity | Human;Mouse;Rat |
| Antigen | β-Tubulin |
| Application | ： ELISA（）1:20,0001:40,000，IF/ICC1:1001:500，IHC1:501:200，WB1:1,0001:5,000。。 |
| Immunogen | beta。，，。 |
| MW | 55kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | SulfoLink™ Coupling Resin was used to purify the antiserum through the process of peptide affinity chromatography. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | To maintain the quality of the product, it is advisable to keep it stored at a temperature of -20 °C for a duration of one year. Ensure that you avoid subjecting the product to repeated freeze/thaw cycles, as it may impact the efficacy of the product. |
| Storage buffer | The provided information is about Rabbit IgG stored in a specific solution. It is stored in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The recommended storage temperature is -20 °C, and it can be kept stable for a period of 12 months starting from the receipt date. |
Target | |
Background | Microtubules, which are composed of tubulin, are an essential part of the cytoskeleton. Tubulin interacts with GTP at two separate sites: the beta chain has an exchangeable site, while the alpha chain has a non-exchangeable site. TUBB3, which is a specific type of tubulin, plays a crucial role in maintaining and guiding axons. |
| Tissue specificity | Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. |
| Posttranslational modification | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
| Celluar localization | Cytoskeleton;Extracellular region or secreted;Nucleus; |
| UniPort | Q13509 |
Western blot analysis of Tubulin beta expression in Mouse Brain, whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
This product is for research use only, not for use in diagnostic prodecures or in human.
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