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Rabbit Anti-CAF1B Polyclonal Antibody#abs133557

Description

Catalog-specification	Delivery time	USD price
abs133557-100ug	1-2 Weeks	301.0
abs133557-50ug	1-2 Weeks	201.0

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Overview
catalog	abs133557
Other names	CAF-1 subunit B, also known as CAF 1A or CAF IP60, is a 60 kDa subunit of the chromatin assembly factor 1 (CAF-1). CAF-1 is involved in the assembly of chromatin, the complex structure of DNA and proteins that make up chromosomes. CAF-1 plays a crucial role in the regulation of gene expression and maintenance of genome stability. The CAF-1 complex consists of three subunits: CAF-1 subunit A (CAF-1A), CAF-1 subunit B (CAF-1B), and CAF-1 subunit C (CAF-1C). Among these subunits, CAF-1B, also referred to as CAF-I 60 kDa subunit or CAF-I p60, is particularly important for its involvement in nucleosome assembly during DNA replication and repair. The human chromatin assembly factor-I p60 subunit, also known as CHAF1B or M-phase phosphoprotein 7 (MPHOSPH7), is encoded by the CAF1B gene. It functions as a critical component of the CAF-1 complex, facilitating the deposition of newly synthesized histones onto replicating DNA strands. This process ensures the faithful inheritance of epigenetic information during cell division. The CAF-1 subunit B (CAF-1B) is often phosphorylated during the M phase of the cell cycle, leading to its alternative name "MPP 7" or "MPP7". This phosphorylation event modulates the activity of CAF-1 and contributes to the proper progression of DNA replication and chromosome segregation. In summary, the CAF-1 subunit B, also known as CAF 1A or CAF IP60, is a key component of the chromatin assembly factor 1 complex involved in nucleosome assembly, DNA replication, and repair. Its phosphorylation during the M phase of the cell cycle further regulates its functions in maintaining genome stability.
Source	Rabbit
Specificity	The CAF1B Antibody is capable of detecting total CAF1B levels that are present within the organism naturally. By utilizing this antibody, researchers can gain important information regarding the presence and function of CAF1B within a given sample. It is crucial to note that the generated content is based on the original text information but is rearranged to provide a highly similar yet unique perspective.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Horse;Pig
Antigen	CAF1B
Application	For Western blotting, we recommend dilutions of 1:500 to 1:1000. For immunofluorescence or immunocytochemistry, a range of 1:100 to 1:500 is appropriate. For ELISA using a peptide antigen, we suggest dilutions of 1:20000 to 1:40000. It is important to use the appropriate dilution for each application in order to obtain optimal results.
Immunogen	A synthesized peptide.
MW	61 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was refined through peptide affinity chromatography. A highly comparable content can be generated through rephrasing the original text information.
Clonality	Polyclonal Antibody
Stability & Storage	The storage temperature for this product is -20°C and it can be safely stored at this temperature for up to one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles to preserve its integrity and quality. To ensure maximum effectiveness, it is recommended to follow the storage instructions carefully.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.
Tissue specificity	Active complex is found in G1, S and G2 phases.
Posttranslational modification	Differentially phosphorylated during cell cycle. During mitosis the p60 subunit of inactive CAF-1 is hyperphosphorylated and displaced into the cytosol. Progressivly dephosphorylated from G1 to S and G2 phase. Phosphorylated p60 is recruited to chromatin undergoing DNA repair after UV irradiation in G1, S or G2 phases.
Celluar localization	Nucleus;
UniPort	Q13112