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Rabbit Anti-STEAP3 Polyclonal Antibody#abs135642

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Description

Catalog-specification	Delivery time	USD price
abs135642-100ug	1-2 Weeks	301.0
abs135642-50ug	1-2 Weeks	201.0

Overview
catalog	abs135642
Description	Caspases play a critical role in regulating apoptosis through different mechanisms. One of the key players among them is Caspase-3, which is responsible for breaking down cellular proteins, leading to the formation of apoptotic bodies and ultimately the dismantling of the cell. The protein pHyde is believed to act as a tumor suppressor by promoting Caspase-3-mediated apoptosis and upregulating the expression of p53. Studies have shown that the introduction of pHyde into DU145 cells results in a dose-dependent increase in Caspase-3 activity, thereby leading to apoptosis. This suggests that the activation of the apoptosis pathway may be one of the key mechanisms through which pHyde acts as a tumor suppressor. Moreover, recombinant pHyde has been found to inhibit the growth of human prostate cancer cell lines such as DU145 and LNCaP in vitro, and also to reduce the size of DU145 tumors in vivo when injected into nude mice. Interestingly, pHyde has also been shown to exhibit growth inhibitory effects on human breast cancer cells, indicating its possible role as a tumor suppressor across different tumor types. Overall, these findings highlight the potential of pHyde as a therapeutic agent in cancer gene therapy by targeting the apoptosis pathway to induce cell death and inhibit tumor growth.
Other names	The protein known as 1010001D01Rik goes by several other names, including Dudlin 2, Dudulin-2, hpHyde, hTSAP6, and MGC93147. It is a metalloreductase belonging to the STEAP family, specifically STEAP3 or STMP3. Additionally, it is known as Tumor suppressor pHyde or Tumor suppressor-activated pathway protein 6. Another name for this protein is Six-transmembrane epithelial antigen of prostate 3 or Six-transmembrane prostate protein 3. It is essential for cell growth and development and has been implicated in the progression of various types of cancer. Despite the many names for this protein, it is clear that it plays a crucial role in cellular processes and warrants further study.
Source	Rabbit
Specificity	The total STEAP3 present in the body can be accurately detected by using the STEAP3 Antibody, which is specifically designed to identify endogenous levels of the protein. To generate similar content, the original text information could be paraphrased as follows: The STEAP3 Antibody is capable of recognizing and measuring the overall quantity of STEAP3 that naturally occurs in the body. This means that it can accurately detect the endogenous levels of STEAP3, and provide valuable insights into its role and function within the body.
Species Reactivity	Human;Mouse;Rat
Antigen	STEAP3
Application	WB: The dilution range for Western blotting is 1:500 to 1:2000. This technique is used to detect specific proteins in biological samples. IHC: For immunohistochemistry, the suggested dilution range is 1:50 to 1:200. This method allows for the localization and visualization of specific antigens in tissue sections. IF/ICC: The recommended dilution range for immunofluorescence or immunocytochemistry is 1:100 to 1:500. This technique enables the detection and imaging of specific proteins or antigens in cells. ELISA (peptide): When performing ELISA with peptide antigens, it is advised to dilute the samples from 1:20000 to 1:40000. This quantitative assay is used to measure the concentration of peptides or proteins of interest in biological samples.
Immunogen	A synthesized peptide derived from human STEAP3.
MW	55kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe3+ to Fe2+. Can also reduce of Cu2+ to Cu1+, suggesting that it participates in copper homeostasis. Uses NADP+ as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.
Tissue specificity	Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.
Posttranslational modification	Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage occurs at multiple sites in a glycosylation-independent manner.Glycosylated.
Celluar localization	Endosome;Plasma Membrane;
UniPort	Q658P3