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LCN2 Antibody#abs136336

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Description

Catalog-specification	Delivery time	USD price
abs136336-50ug	1-2 Weeks	201.0
abs136336-100ug	1-2 Weeks	301.0

Overview
catalog	abs136336
Description	Lipocalin-2 is a protein that belongs to the lipocalin family and was first discovered as a gelatinase-associated component of neutrophil secretory granules. Its functions are multiple and diverse, encompassing innate immunity, iron homeostasis, and apoptosis. One of its roles in the body is to combat bacterial growth by binding to bacterial siderophores and removing the iron, thus limiting bacterial growth. When loaded with iron, lipocalin-2 binds to its receptor and is internalized, releasing the iron and increasing the intracellular iron concentration. Conversely, when iron-free, lipocalin-2 promotes apoptosis. Interestingly, lipocalin-2 has also been found to be expressed in adipose tissue, where it plays a role in promoting insulin resistance in cultured mouse adipocytes. Overall, the multifaceted features of lipocalin-2 make it a critical player in many physiological processes.
Other names	The protein known as lipocalin-2, or Lcn2, is also referred to as neutrophil gelatinase-associated lipocalin, or NGAL. It is a 25 kDa alpha-2-microglobulin-related subunit of matrix metalloproteinase 9 (MMP-9). Lcn2 is involved in a variety of physiological processes, including the regulation of iron homeostasis and inflammation. It is also associated with various pathological conditions, such as cancer, cardiovascular disease, and kidney injury. Additionally, Lcn2 has been identified as a migration stimulating factor inhibitor and is known to bind to bacterial siderophores, leading to its alternative name of siderocalin. Despite its many roles, the exact mechanisms and pathways through which lipocalin-2 operates are still being studied.
Source	Rabbit
Specificity	Endogenous levels of total LCN2 can be accurately identified using the LCN2 Antibody.
Species Reactivity	Human;Mouse;Rat
Antigen	LCN2
Application	For Western blotting, you can dilute your primary antibody at a range of 1:500 to 1:2000. When it comes to immunohistochemistry, dilution rates of 1:50 to 1:200 are most appropriate. If you are analyzing peptide samples using ELISA, dilution rates ranging from 1:20,000 to 1:40,000 should give you the desired results. It's important to follow the recommended dilution guidelines in order to obtain accurate and reliable data.
Immunogen	A synthesized peptide derived from human LCN2.
MW	23kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
Tissue specificity	Expressed in bone marrow and in tissues that are prone to exposure to microorganism. High expression is found in bone marrow as well as in uterus, prostate, salivary gland, stomach, appendix, colon, trachea and lung. Not found in the small intestine or peripheral blood leukocytes.
Celluar localization	Cytosol;Extracellular region or secreted;
UniPort	P80188