Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-MKI67 Polyclonal Antibody#abs136338

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Western blot analysis of extracts of HeLacell lines, using MKI67 antibody. This product is for research use only, not for use in...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs136338-100ug	1-2 Weeks	301.0
abs136338-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent.

Overview
catalog	abs136338
Description	Ki-67 is a nonhistone protein found in the nucleus, discovered in Kiel University, Germany. It is expressed in all actively dividing cells but not in cells that are resting. Hence, it's an efficient marker to detect proliferating cells in various phases of the cell cycle. Studies have shown that high levels of Ki-67 are linked to poor survival rates in patients with breast cancer. Researchers have also explored Ki-67 as a potential prognostic or predictive marker for various malignancies, including breast cancer. This marker, when combined with other markers, can provide valuable information for the management of patients with breast cancer.
Other names	The Ki-67 antigen is identified by the monoclonal antibody Ki-67 and is also known as the proliferation marker protein Ki-67. Other aliases for this antigen include MIB-1, KIA, MKI67, PPP1R105, and RP11-380J17.2. It is a marker used to track cell proliferation and has been extensively studied in cancer research. The antigen Ki-67 is important for determining the growth fraction of cells and can be used to predict the prognosis of various cancers. It is a valuable tool for researchers and clinicians in understanding the growth patterns of tumors and developing treatment strategies.
Source	Rabbit
Specificity	The MKI67 Antibody can identify the total amount of endogenous MKI67 present in a sample. To create similar content, the information from the original text can be rearranged and structured differently. It is important to use a language model that generates text in a completely different manner than ChapGPT to ensure the authenticity of the generated content.
Species Reactivity	Human;Rat
Antigen	MKI67
Application	The recommended dilutions for different assays are as follows: for western blotting, use a dilution range of 1:500 to 1:2000; for immunohistochemistry, use a dilution range of 1:50 to 1:200; and for ELISA with peptide antigens, use a dilution range of 1:20000 to 1:40000. It is crucial to follow the recommended dilution ranges for optimal assay performance and accurate results. Dilution factors outside these ranges may result in non-specific binding, high background noise or weak signals. Therefore, it is advisable to optimize the assay conditions for a specific application.
Immunogen	A synthesized peptide derived from human MKI67.
MW	359kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLinkâ„¢ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. A highly similar content can be created by reorganizing the original text while still retaining the vital information.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 Â°C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 Â°C.Stable for 12 months from date of receipt.
Target
Background	Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Tissue specificity	Expression occurs preferentially during late G1, S, G2 and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected (at protein level) (PubMed:6206131). Present at highest level in G2 phase and during mitosis (at protein level). In interphase, forms fiber-like structures in fibrillarin-deficient regions surrounding nucleoli (PubMed:2674163, PubMed:8799815).
Posttranslational modification	Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
Celluar localization	Nucleus;
UniPort	P46013