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Rabbit Anti-ACTA1/A2/C1 Polyclonal Antibody#abs133032

Description

Catalog-specification	Delivery time	USD price
abs133032-100ug	1-2 Weeks	301.0
abs133032-50ug	1-2 Weeks	201.0

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Overview
catalog	abs133032
Description	Actins, which are ubiquitously expressed in all eukaryotic cells, play a crucial role in diverse forms of cell motility. These highly conserved proteins are involved in various cellular activities.
Other names	Actin is a protein that makes up the cytoskeleton of cells and plays a vital role in various cellular processes, including cell division and movement. There are several types of actin, including ACTA, ACTA1, ACTA2, ACTC, and ACTC1, each with its specific function. Alpha 2 actin, also known as AAT6, is a skeletal muscle protein that works primarily in muscle contraction. It is composed of two distinct subunits, alpha and beta, which join together to form the active muscle protein. Cardiac muscle alpha actin 1 is another type of actin that is primarily found in heart muscle cells. It helps to regulate and maintain the intricate structure of cardiac muscle tissue, ensuring proper functioning of the heart. Actin alpha 2 smooth muscle aorta is a type of actin that is found primarily in the smooth muscle of the aorta. It plays a crucial role in the contraction and relaxation of smooth muscle cells, thereby controlling blood flow and blood pressure. In summary, actin is a vital protein that helps maintain the cytoskeleton of cells, enabling them to function correctly. Its various types and functions make it an essential component of muscle tissue and ensure proper blood flow throughout the body.
Source	Rabbit
Specificity	The ACTA1/A2/C1 antibody has the ability to identify the entire pool of ACTA1/A2/C1 proteins that are present within a biological sample. This antibody has been designed to target the endogenous levels of ACTA1/A2/C1, which ensures that it can accurately distinguish between different forms and isoforms of this protein family. By detecting total ACTA1/A2/C1 protein levels, this antibody can provide valuable insight into the regulation and activity of these proteins within cells and tissues.
Species Reactivity	Human;Mouse;Rat
Antigen	ACTA1/A2/C1
Application	WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide.
MW	42 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, we purified the antiserum through peptide affinity chromatography. The process resulted in a highly purified antiserum, free from impurities that may affect the accuracy or quality of test results. Our aim was to develop a reliable and trustworthy antiserum that would provide consistent and dependable results. Overall, we are confident that our purification process has achieved this goal and produced a high-quality antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Target
Background	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Posttranslational modification	Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization (By similarity).Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).
Celluar localization	Cytoskeleton;Cytosol;Extracellular region or secreted;
UniPort	P68133/P62736/P68032