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Rabbit Anti-TNF12 Polyclonal Antibody#abs133325

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Description

Catalog-specification	Delivery time	USD price
abs133325-100ug	1-2 Weeks	301.0
abs133325-50ug	1-2 Weeks	201.0

Overview
catalog	abs133325
Other names	Tumor necrosis factor superfamily member 12, also known as TNFSF12 or APO3 ligand, is a protein that plays a role in apoptosis and immune regulation. It is encoded by the APO3L gene and exists in both membrane-bound and secreted forms. Other names for this protein include APO 3L, APO3/DR3 ligand, DR3LG, MGC129581, MGC20669, TNF-related weak inducer of apoptosis, TNFSF12 protein, and TWEAK. APO3 ligand is a key player in the TNF superfamily, exerting its effects through interaction with its receptor, DR3. The binding of APO3 ligand to DR3 triggers various signaling pathways that can lead to either cell survival or apoptosis, depending on the cellular context. This ligand-receptor interaction has been implicated in the regulation of immune responses, inflammation, tissue repair, and cancer. The APO3 ligand can be found in both soluble and membrane-bound forms. The soluble form is generated by proteolytic cleavage and can act as a cytokine. It has been shown to induce the production of pro-inflammatory cytokines, promote cell migration, and enhance angiogenesis. On the other hand, the membrane-bound form of APO3 ligand is involved in cell-cell interactions and can mediate cell death through receptor-ligand crosslinking. In summary, APO3 ligand is a multifunctional protein that belongs to the TNF superfamily. Its involvement in apoptosis and immune regulation makes it an important player in various physiological and pathological processes. Further research into the signaling pathways and functional consequences of APO3 ligand activation may reveal potential therapeutic targets for the treatment of immune-related disorders and cancer.
Source	Rabbit
Specificity	The TNF12 Antibody has the ability to identify total TNF12 present in the body. This detection is aimed at detecting the natural levels of TNF12 and any changes occurring in them.
Species Reactivity	Human;Mouse
Antigen	TNF12
Application	The dilution ratios for Western Blotting range from 1:500 to 1:1000, for Immunohistochemistry it ranges from 1:50 to 1:200, for Immunofluorescence/Immunocytochemistry it ranges from 1:100 to 1:500, and for ELISA (peptide) it ranges from 1:20000 to 1:40000. Please note that the content generated here is in a different format from the original text, as per the specific instructions given.
Immunogen	A synthesized peptide.
MW	27 KD
Properties
Concentration	1mg/ml
purification	To obtain a potent antiserum, peptide affinity chromatography was employed, and the SulfoLink™ Coupling Resin was used for purification.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain quality, it is recommended to store at a temperature of -20 °C for a period of one year. Make sure to avoid subjecting the product to repeated freeze/thaw cycles to preserve efficacy.
Storage buffer	This product is Rabbit IgG in phosphate buffered saline, pH 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store it at -20 °C. The stability of this product is guaranteed for up to 12 months from the date of receipt.
Target
Background	Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion.
Tissue specificity	Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.
Posttranslational modification	The soluble form derives from the membrane form by proteolytic processing.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	O43508