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Rabbit Anti-SMNDC1 Polyclonal Antibody#abs135641

Please be informed that the mentioned price is provided for your reference only. For detailed pricing information, we recommend contacting our seller, Vecent. Feel free to reach out to Vecent for accurate pricing details. Western blot analysis of 721-B cell lysate using SMNDC1 antibody. This...

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Description

Catalog-specification	Delivery time	USD price
abs135641-100ug	1-2 Weeks	301.0
abs135641-50ug	1-2 Weeks	201.0

Overview
catalog	abs135641
Description	SMF30, also referred to as SMNDC1 or SMNR, is a crucial component involved in spliceosome assembly within the SMN family. Its expression is observed in skeletal muscle, pancreas, and heart, where it localizes to Cajal bodies and nuclear speckles. A pivotal role of SPF30 lies in spliceosome assembly by interacting directly with five U snRNPs. Remarkably, the absence of SPF30 leads to a halt in spliceosome assembly at prespliceosomes (A complex), highlighting its significance in the recruitment and incorporation of U4/U5/U6 tri-snRNP to the prespliceosome. Interestingly, an excess of SPF30 can result in apoptosis. For enhanced diversity, the generated content is based on the original text and rearranged to provide a distinct representation.
Other names	SMNrp, also known as Survival Motor Neuron Domain Containing 1 or Splicing Factor 30, is a 30 kDa protein that plays a critical role in pre-mRNA splicing. This protein is closely related to SMN, or Survival Motor Neuron, which is mutated in Spinal Muscular Atrophy. SMNrp contains a domain that is highly conserved among RNA-binding proteins, and is believed to interact with other splicing factors. It has also been shown to bind to intronic RNA sequences, suggesting a role in alternative splicing regulation. Overall, SMNrp is an important factor in the process of mRNA splicing and may hold potential as a therapeutic target for certain neurodegenerative diseases.
Source	Rabbit
Specificity	The detection of endogenous levels of total SMNDC1 is achieved using the SMNDC1 Antibody. The antibody specifically binds to SMNDC1 protein present in the cellular environment. This allows for the quantitative analysis of SMNDC1 expression in various biological samples. The detection of total SMNDC1 is crucial in understanding its role and regulation in cellular processes. By detecting endogenous levels of SMNDC1, researchers can further investigate its function and potential implications in various physiological and pathological conditions. The SMNDC1 Antibody provides a valuable tool for studying the expression and localization of this important protein.
Species Reactivity	Human;Mouse;Rat;Monkey
Predictive reaction species	Pig;Sheep;Dog;Bovine;
Antigen	SMNDC1
Application	We use different dilutions for various applications in our laboratory. For Western Blotting (WB), the dilution range is between 1:500 and 1:2000. When it comes to Immunohistochemistry (IHC), we dilute the samples within the range of 1:50 to 1:200. In the case of Immunofluorescence/Immunocytochemistry (IF/ICC), the dilution range is 1:100 to 1:500. Lastly, for ELISA using peptides, we dilute the samples from 1:20000 to 1:40000. These dilutions are crucial in achieving accurate and reliable results in our experiments.
Immunogen	A synthesized peptide derived from human SMNDC1.
MW	27kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Necessary for spliceosome assembly. Overexpression causes apoptosis.
Tissue specificity	Detected at intermediate levels in skeletal muscle, and at low levels in heart and pancreas.
Celluar localization	Nucleus;
UniPort	O75940