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Rabbit Anti-SOAT1 Polyclonal Antibody#abs137255

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Description

Catalog-specification	Delivery time	USD price
abs137255-100ug	1-2 Weeks	301.0
abs137255-50ug	1-2 Weeks	201.0

Overview
catalog	abs137255
Other names	ACACT; ACAT; ACAT-1; ACAT1; Acyl coenzyme A cholesterol acyltransferase 1; Acyl-coenzyme A:cholesterol acyltransferase 1; Cholesterol acyltransferase 1; SOAT; SOAT1; SOAT1_HUMAN; STAT; Sterol O acyltransferase 1; Sterol O-acyltransferase 1; I am here to provide information on ACAT1, also known as Acyl coenzyme A cholesterol acyltransferase 1 or Cholesterol acyltransferase 1. This enzyme, coded by the gene ACAT1, plays a crucial role in the metabolism of cholesterol in the human body. ACAT1, sometimes referred to as ACAT or ACAT-1, is responsible for the synthesis of cholesterol esters. These esters are important for the transport and storage of cholesterol within cells. The enzyme transfers fatty acids from coenzyme A to cholesterol, converting it into cholesterol esters. Additionally, ACAT1 is also known as SOAT1 (Sterol O-acyltransferase 1). This alternative name highlights its function in catalyzing the esterification of sterols, including cholesterol. By adding an acyl group to cholesterol, ACAT1 assists in its packaging into lipoprotein particles for transportation in the bloodstream. The ACAT1 enzyme is widely expressed in various tissues, including the liver, intestine, and macrophages. Its activity is tightly regulated to maintain cholesterol homeostasis in the body. Dysregulation of ACAT1 has been associated with various diseases, such as atherosclerosis and Alzheimer's disease. In summary, ACAT1, also known as SOAT1 or Sterol O-acyltransferase 1, is an essential enzyme involved in cholesterol metabolism. Its function in synthesizing cholesterol esters and facilitating cholesterol transport makes it a crucial player in maintaining cellular cholesterol balance.
Source	Rabbit
Specificity	Total SOAT1 levels can be detected by the SOAT1 Antibody, which specifically targets endogenous amounts of the protein. To maintain the original text information, the content has been rephrased and rearranged.
Species Reactivity	Human;Rat
Predictive reaction species	Sheep;Bovine;Dog;Horse;
Antigen	SOAT1
Application	IHC 1:200, WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000. The various antibody dilutions mentioned above indicate the recommended concentrations for different experimental techniques. WB stands for Western Blot, where the typical range for antibody dilution is between 1:1000 and 1:3000. IHC refers to Immunohistochemistry, and the recommended antibody dilution for this technique is 1:200. Lastly, ELISA (peptide) is another experimental method, and the antibody dilution range for this specific ELISA technique is between 1:20000 and 1:40000. It is important to note that these dilutions are guidelines and may need to be further optimized based on the specific experiment and antibody characteristics.
Immunogen	A synthesized peptide derived from human SOAT1.
MW	65 kDa
Properties
Concentration	1mg/ml
purification	The purification of the antiserum involved the use of SulfoLink™ Coupling Resin for peptide affinity chromatography. This process allowed for the highly efficient purification of the antiserum and ensured that it was properly cleaned and separated from other compounds.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain its quality, keep the product stored at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to repeated cycles of freezing and thawing.
Storage buffer	The provided information pertains to Rabbit IgG, which is stored in phosphate buffered saline with a pH of 7.4. It contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store the product at -20 °C, and it remains stable for a period of 12 months from the date of receipt.
Target
Background	Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase.
Tissue specificity	Highly activated by the presence of cholesterol.
Celluar localization	Endoplasmic reticulum;
UniPort	P35610