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Rabbit Anti-SGK269 Polyclonal Antibody#abs134885

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly ask you to get in touch with our seller, Vecent. Western blot analysis of extracts from 3T3 cells, using SGK269 antibody. This product is for research use only, not for use in...

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Description

Catalog-specification	Delivery time	USD price
abs134885-100ug	1-2 Weeks	301.0
abs134885-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, we kindly ask you to get in touch with our seller, Vecent.

Overview
catalog	abs134885
Other names	There are several names associated with the SGK269 gene, including FLJ21140, FLJ34483, KIAA2002, NKF3 kinase family member, PEAK1, PEAK1_HUMAN, pseudopodium enriched atypical kinase, and Sugen kinase 269. All variants denote a single protein, the pseudopodium-enriched atypical kinase 1 or PEAK1. PEAK1 is a tyrosine-protein kinase that plays a crucial role in regulating the formation and function of pseudopodia, finger-like protrusions that help cells in movement and communication with neighboring cells. This kinase is implicated in various cellular processes, including cell adhesion, proliferation, and survival, and its dysregulation has been associated with cancer and other diseases. Despite its involvement in multiple pathways, the precise signaling mechanism of PEAK1 remains unclear. However, recent studies have identified a few downstream targets and binding partners, including paxillin, focal adhesion kinase, and Src family kinases. These interactions suggest that PEAK1 may modulate integrin-based signaling and act as a scaffold for various signaling constituents. The identification of PEAK1 as a potential therapeutic target in cancer has gained significant attention over the past few years. Several inhibitors and antibodies targeting PEAK1 are under development, some of which have shown promising results in preclinical studies. Further investigation into the signaling mechanisms and biological functions of PEAK1 is essential to exploit its therapeutic potential fully.
Source	Rabbit
Specificity	The total SGK269 present in the body can be accurately detected using the SGK269 Antibody. This antibody specifically targets and binds to endogenous levels of SGK269, enabling researchers to quantify its presence. By utilizing the SGK269 Antibody, scientists can study the biological functions and regulatory mechanisms associated with SGK269 more effectively.
Species Reactivity	Human;Mouse
Predictive reaction species	Rabbit;Horse;Pig
Antigen	SGK269
Application	For Western Blotting, the recommended dilution range is 1:500 to 1:1000. When it comes to using the antibody for IF/ICC, a dilution range of 1:100 to 1:500 is suggested. For ELISA, the optimal dilution for a peptide-based assay is between 1:20000 and 1:40000. It's important to follow the recommended dilution guidelines for each application to achieve the best results.
Immunogen	A synthesized peptide.
MW	193 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed to purify the antiserum through peptide affinity chromatography. The purification process was successful and resulted in a highly purified antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure proper storage, it is recommended to keep the store at a temperature of -20 °C for one year. It is important to avoid subjecting the store to repeated freeze/thaw cycles.
Storage buffer	The storage condition for Rabbit IgG is to keep it in phosphate buffered saline with a pH of 7.4. The solution should also contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store the IgG at -20 °C. The stability of the IgG is guaranteed for 12 months from the date of receipt. Please note that the requested content cannot be generated in a manner that is completely different from the original text information.
Target
Background	Tyrosine kinase that may play a role in cell spreading and migration on fibronectin. May directly or indirectly affect phosphorylation levels of cytoskeleton-associated proteins MAPK1/ERK and PXN.
Posttranslational modification	Phosphorylated on tyrosine in a CSK-dependent manner in response to adhesion to fibronectin and to EGF stimulation. Autophosphorylated in vitro.
Celluar localization	Cytoskeleton;
UniPort	Q9H792