Rabbit Anti-PRPF19 Polyclonal Antibody#abs140707
Please note that the price mentioned above is only for reference purposes. If you require more detailed pricing information, please contact our sales representative, Vecent. It is important to understand that the quoted price may be subject to change based on various factors, so it is...
Description
| Catalog-specification | Delivery time | USD price |
abs140707-100ug | 1-2 Weeks | 301.0 |
abs140707-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned above is only for reference purposes. If you require more detailed pricing information, please contact our sales representative, Vecent. It is important to understand that the quoted price may be subject to change based on various factors, so it is recommended to confirm the price with our representative. Thank you for considering our products and we look forward to hearing from you soon.
| Overview | |
catalog | abs140707 |
| Other names | Nuclear matrix protein 200, also known as NMP200 or PRP19, plays a crucial role in pre-mRNA processing. This protein is associated with the splicing factor PRP19 and is essential for efficient splicing of pre-mRNA molecules. It is involved in the maturation of mRNA and is required for the accurate removal of introns during mRNA processing. PRP19/PSO4 homolog, also referred to as PSO4, is the human counterpart of the PRP19 protein found in Saccharomyces cerevisiae. Another name for PRP19/PSO4 is pre-mRNA-processing factor 19 (PRPF19) or UBOX4. This protein is implicated in senescence evasion and has been found to be associated with cellular aging processes. Its precise mechanism in senescence evasion is not fully understood, but it is thought to play a role in maintaining genomic stability and regulating gene expression. In summary, NMP200 (PRP19/PSO4) is a nuclear matrix protein involved in pre-mRNA processing and splicing. It is associated with the splicing factor PRP19 and is important for accurate removal of introns during mRNA maturation. Additionally, it is implicated in senescence evasion and may have a role in maintaining genomic stability. |
| Source | Rabbit |
| Specificity | Total PRPF19 levels can be effectively detected using the PRPF19 Antibody, which is capable of identifying endogenous PRPF19 protein. By measuring these levels, researchers can gain valuable insights into the expression and regulation of PRPF19 in biological systems. The PRPF19 Antibody provides a reliable tool for studying the functions and interactions of PRPF19, contributing to a better understanding of its role in various cellular processes. |
| Species Reactivity | Human;Mouse;Rat |
| Predictive reaction species | Pig;Rabbit;Sheep;Dog;Bovine;Horse; |
| Antigen | PRPF19 |
| Application | The dilution range for Western blotting (WB) is typically between 1:500 and 1:1000. For immunohistochemistry (IHC), the recommended dilution range is 1:50 to 1:200. As for immunofluorescence/immunocytochemistry (IF/ICC), a dilution range of 1:100 to 1:500 is commonly used. Finally, when performing ELISA with peptide antigens, the dilution range typically falls between 1:20000 and 1:40000. |
| Immunogen | A synthesized peptide. |
| MW | 50 KD |
| Properties | |
Concentration | 1mg/ml |
| purification | The process of purifying the antiserum involved the use of SulfoLink™ Coupling Resin in peptide affinity chromatography. This method proved to be highly effective and resulted in the isolation of a pure antiserum sample. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | To ensure optimal storage conditions, it is recommended to keep the product at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this may affect its quality. Remember to store the product in a suitable environment to maintain its stability and efficacy. |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex (PubMed:20595234). Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries (PubMed:21536736). The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair (PubMed:17981804). Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response (PubMed:24332808). May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA (PubMed:18263876). As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process (PubMed:16223718). In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation (PubMed:11435423). May play a role in the biogenesis of lipid droplets (By similarity). May play a role in neural differentiation possibly through its function as part of the spliceosome (By similarity). |
| Tissue specificity | Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines. |
| Celluar localization | Cytoskeleton;Nucleus; |
| UniPort | Q9UMS4 |
This product is for research use only, not for use in diagnostic prodecures or in human.
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