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Rabbit Anti-FAS Ligand Polyclonal Antibody#abs131431

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Description

Catalog-specification	Delivery time	USD price
abs131431-100ug	1-2 Weeks	301.0
abs131431-50ug	1-2 Weeks	201.0

Overview
catalog	abs131431
Description	FAS ligand is a type of cytokine that can bind to TNFRSF6/FAS, which is a receptor responsible for transmitting the apoptotic signal into cells. In particular, this cytokine may play a role in T-cell development and apoptosis mediated by cytotoxic T-cells. In addition, the TNFRSF6/FAS-mediated apoptosis may be involved in inducing peripheral tolerance. These findings suggest that FAS ligand may have important implications for understanding the immune system and mediating immune responses.
Other names	The ALPS1B gene is responsible for encoding the Fas ligand, which is a member of the tumor necrosis factor (TNF) superfamily. This protein is also known as CD95-L, CD178, or TNFSF6. Its main function is to trigger apoptosis (cell death) when it binds to its receptor, Fas, on the surface of cells. This process is important for the immune system to eliminate damaged or infected cells. Mutations in the ALPS1B gene can lead to generalized lymphoproliferative disease (Gld), which is a rare genetic disorder characterized by abnormal lymphocyte proliferation and autoimmune disease. There is also a soluble form of the Fas ligand that can be produced and released by cells. Overall, the Fas ligand plays a crucial role in regulating immune system function and maintaining tissue homeostasis. Its dysregulation can lead to various pathologies, making it a target for therapeutic interventions.
Source	Rabbit
Specificity	The total FAS ligand levels can be detected by using an antibody specific to FAS ligand, enabling the measurement of endogenous levels of FAS ligand.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig
Antigen	FAS ligand
Application	The recommended dilutions for WB range from 1:500 to 1:2000, while for IHC, it is 1:50 to 1:200. For ELISA (peptide), the ideal dilution is between 1:20000 to 1:40000. It is important to carefully follow these guidelines to ensure accurate and reproducible results. Dilutions that deviate significantly from these ranges may lead to weaker signals or increased background noise, making it difficult to draw meaningful conclusions from experimental data. By optimizing dilutions within these ranges, researchers can achieve reliable and consistent outcomes in their experiments.
Immunogen	FAS。，，。，。 FAS。
MW	31 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized for the purification of the antiserum through peptide affinity chromatography. The resulting product was highly purified and of excellent quality, ensuring its suitability for various applications.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:26334989, PubMed:9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:9228058, PubMed:7528780, PubMed:9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed:27806260).
Posttranslational modification	The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10 producing an N-terminal fragment, which lacks the receptor-binding extracellular domain. This ADAM10-processed FasL (FasL APL) remnant form is still membrane anchored and further processed by SPPL2A that liberates the FasL intracellular domain (FasL ICD). FasL shedding by ADAM10 is a prerequisite for subsequent intramembrane cleavage by SPPL2A in T-cells.N-glycosylated (PubMed:9228058). Glycosylation enhances apoptotic activity (PubMed:27806260).Phosphorylated by FGR on tyrosine residues; this is required for ubiquitination and subsequent internalization.Monoubiquitinated.
Celluar localization	Extracellular region or secreted;Lysosome;Nucleus;Plasma Membrane;
UniPort	P48023