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Rabbit Anti-PI3 Kinase P85 Beta Polyclonal Antibody#abs133286

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Description

Catalog-specification	Delivery time	USD price
abs133286-100ug	1-2 Weeks	301.0
abs133286-50ug	1-2 Weeks	201.0

Overview
catalog	abs133286
Other names	The P85 regulatory subunit of phosphatidylinositol 3 kinase is a crucial component in signal transduction pathways. Specifically, the beta isoform of P85, also known as PIK3R2, plays a key role in regulating the enzyme activity of PI3K. This regulatory subunit is essential for activating the catalytic subunit, thereby enabling PI3K to catalyze the conversion of phosphatidylinositol-4,5-bisphosphate (PIP2) to phosphatidylinositol-3,4,5-trisphosphate (PIP3). PIP3 is a crucial second messenger that triggers downstream signaling cascades involved in cell growth, survival, proliferation, and metabolism. The beta isoform of P85 is widely expressed in various tissues and cell types, highlighting its importance in regulating diverse physiological processes.
Source	Rabbit
Specificity	The PI 3 Kinase p85 beta Antibody is able to identify total amounts of endogenous PI 3 Kinase p85 beta. This antibody is capable of detecting levels of the protein within cells and tissues, and can be used for a variety of research applications. By recognizing this particular isoform of PI 3 kinase, scientists can gain insight into various biological processes, including cell signaling cascades and metabolic pathways. Overall, this antibody is a valuable tool for researchers studying the role of PI 3 Kinase p85 beta in various cellular processes.
Species Reactivity	Human;Mouse;Rat
Antigen	PI3 Kinase p85 β
Application	The recommended dilutions for WB are 1:1000-3000, for IHC 1:200, for ICC/IF, and ELISA (peptide) the dilutions range from 1:20000-1:40000. It is important to follow these guidelines to achieve accurate results in your experiments. Proper dilution ensures that the antibodies are used at an optimal concentration for detecting the target protein.
Immunogen	A peptide has been synthesized, which is obtained from the human PI 3 Kinase p85 beta. The content generated is very similar to the original text by rearranging the words and sentences. This peptide is a highly similar copy of the original human PI 3 Kinase p85 beta.
MW	82kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Regulatory subunit of phosphoinositide-3-kinase (PI3K), a kinase that phosphorylates PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Binds to activated (phosphorylated) protein-tyrosine kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Indirectly regulates autophagy (PubMed:23604317). Promotes nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (By similarity).
Posttranslational modification	Phosphorylated in response to signaling from activated receptor-type protein kinases (PubMed:19690332, PubMed:20068231). Dephosphorylated by PTPRJ (PubMed:18348712). Dephosphorylated at Tyr-655 by PTPN13. Phosphorylation of Tyr-655 impairs while its dephosphorylation promotes interaction with FBXL2 and SCF(FBXL2)-mediated polyubiquitination (PubMed:23604317).Ubiquitinated. Polyubiquitination by the SCF(FBXL2) complex probably promotes proteasomal degradation of PIK3R2.
Celluar localization	Cytosol;Nucleus;
UniPort	O00459

Western blot analysis PI 3 Kinase p85 beta using HUVEC whole cell lysates

This product is for research use only, not for use in diagnostic prodecures or in human.

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