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Rabbit Anti-NFATC1 Polyclonal Antibody#abs135966

Description

Catalog-specification	Delivery time	USD price
abs135966-100ug	1-2 Weeks	301.0
abs135966-50ug	1-2 Weeks	201.0

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Overview
catalog	abs135966
Description	The NFAT (nuclear factor of activated T cells) protein family, comprising NFAT1 (NFATc2 or NFATp), NFAT2 (NFATc1 or NFATc), NFAT3 (NFATc4), and NFAT4 (NFATc3 or NFATx), consists of transcription factors that work in conjunction with AP-1 (Jun/Fos) to regulate gene transcription for an effective immune response (1,2). These proteins are primarily found in immune cells but also play a role in regulating cell differentiation in skeletal muscle, keratinocytes, and adipocytes (3). In their inactive state, NFAT proteins are phosphorylated and located in the cytoplasm. When calcium levels rise, the calcium/calmodulin-dependent serine phosphatase calcineurin is activated and dephosphorylates NFAT proteins, allowing them to enter the nucleus (2). NFAT signaling ceases when calcium levels drop and NFAT is phosphorylated by kinases like GSK-3 or CK1 (3,4). To inhibit calcineurin and prevent NFAT proteins from translocating to the nucleus, immunosuppressive drugs like cyclosporin A and FK506 are used, which keep NFAT proteins in the cytoplasm (5).
Other names	NFATc1, also known as Nuclear factor of activated T cells cytoplasmic 1, is an essential transcription factor involved in immune cell activation and differentiation. It is a cytoplasmic protein that translocates to the nucleus upon activation, where it binds to specific DNA sequences and regulates the expression of target genes. NFATc1, also referred to as NF-ATc or Nuclear factor of activated T-cells 'c', plays a critical role in the immune response and plays an essential role in the differentiation of various cell types. It has been identified as a key player in the regulation of T cell activation and differentiation, as well as in the development of certain cancers. The translocation of NFATc1 from the cytoplasm to the nucleus is regulated by Ca2+/calcineurin signaling, which is activated following T cell receptor activation. Once in the nucleus, NFATc1 binds to DNA and functions as a transcription factor, regulating the expression of genes involved in T cell activation and differentiation. NFATc1 is a highly conserved protein, and homologs of NF-ATc have been identified in many different species, including mice, rats, and humans. Despite its importance in immune function, the precise role of NFATc1 in diverse cellular processes is still being elucidated by researchers worldwide.
Source	Rabbit
Specificity	The detection of total NFATC1 at endogenous levels is achieved using NFATC1 Antibody. Let's generate a highly similar content by rearranging the information from the original text: NFATC1 Antibody is a reliable tool that allows for the identification and quantification of endogenous NFATC1 protein levels. This antibody is designed to specifically recognize total NFATC1, providing accurate and sensitive measurements. With the ability to detect endogenous NFATC1, researchers can gain valuable insights into its expression and activation in various biological processes.
Species Reactivity	Human;Mouse;Rat
Antigen	NFATC1
Application	There are a few different recommended dilution ratios for using various assays with our product. For Western Blotting, we suggest using a dilution range of 1:500 to 1:2000. When performing Immunohistochemistry, we recommend a dilution range of 1:50 to 1:200. Finally, for ELISA assays using peptide, a dilution range of 1:20000 to 1:40000 is suggested. It's important to carefully follow the recommended dilution ranges when using our product to ensure accurate and reliable assay results.
Immunogen	A synthesized peptide derived from human NFATC1.
MW	78kDa;101kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription. Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells (PubMed:10358178). Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function (By similarity).
Tissue specificity	Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure. Isoforms IA are widely expressed but not detected in liver nor pancreas, neural expression is strongest in corpus callosum. Isoforms IB are expressed mostly in muscle, cerebellum, placenta and thymus, neural expression in fetal and adult brain, strongest in corpus callosum.
Posttranslational modification	Phosphorylated by NFATC-kinase and GSK3B; phosphorylation induces NFATC1 nuclear exit and dephosphorylation by calcineurin promotes nuclear import. Phosphorylation by PKA and DYRK2 negatively modulates nuclear accumulation, and promotes subsequent phosphorylation by GSK3B or casein kinase 1.
Celluar localization	Cytosol;Nucleus;
UniPort	O95644