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Rabbit Anti-RCC1 Polyclonal Antibody#abs137752

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our sales representative, Vecent. Western blot analysis RCC1 using RAW264.7 whole cell lysates This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs137752-100ug	1-2 Weeks	301.0
abs137752-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is only for your reference. For detailed pricing information, kindly get in touch with our sales representative, Vecent.

Overview
catalog	abs137752
Other names	RCC1, also known as regulator of chromosome condensation 1 or chromosome condensation protein 1, is a crucial protein involved in the regulation of the cell cycle. It functions as a guanine nucleotide releasing protein, promoting the release of guanine nucleotides from the protein Ran and thereby regulating its activity. Additionally, RCC1 is responsible for the condensation of chromosomes during cell division. Another name for RCC1 is CHC1 (chromosome condensation 1), which is derived from its role in condensing chromosomes. This protein is encoded by the HERC2 gene and is also referred to as Ran GEF or RanGEF, highlighting its function as a regulator of the small GTPase Ran. RCC1 is a fundamental protein in cell division and is involved in various cellular processes. It is essential for proper chromosome condensation and segregation, ensuring accurate distribution of genetic material during cell division. Interestingly, there is a long non-coding RNA called SNHG3 RCC1, which is transcribed from the SNHG3 RCC1 readthrough transcript. These RNA molecules might have regulatory roles in conjunction with RCC1, possibly influencing its expression or activity. To summarize, RCC1 is a vital cell cycle regulatory protein that contributes to chromosome condensation and accurate distribution of genetic material during cell division. Its multifaceted functions make it a key player in maintaining genome stability.
Source	Rabbit
Specificity	The antibody RCC1 can detect total levels of endogenous RCC1 in cells. This means that the antibody can accurately recognize and bind to the protein RCC1, allowing for detection of its presence in the cell. Overall, this information is important for studying various cellular processes that involve RCC1 and its interactions with other proteins and molecules.
Species Reactivity	Human;Mouse;Rat
Antigen	RCC1
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human RCC1.
MW	45 kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography was employed to purify the antiserum using SulfoLink™ Coupling Resin.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. To prevent any damage to the product, it is important to avoid any repeated cycles of freezing and thawing. Therefore, it is important to handle the product with care and adhere to the recommended storage conditions to ensure its efficacy and potency.
Storage buffer	The rabbit IgG is contained in phosphate buffered saline, with a pH level of 7.4, and includes 150 millimolar of NaCl, 0.02% sodium azide, and 50% glycerol. When stored at a temperature of -20 degrees Celsius, this substance can remain stable for up to 12 months from the date of receipt.
Target
Background	Guanine-nucleotide releasing factor that promotes the exchange of Ran-bound GDP by GTP. Involved in the regulation of onset of chromosome condensation in the S phase. Binds both to the nucleosomes and double-stranded DNA. RCC1-Ran complex (together with other proteins) acts as a component of a signal transmission pathway that detects unreplicated DNA. Plays a key role in nucleo-cytoplasmic transport, mitosis and nuclear-envelope assembly.
Posttranslational modification	N-terminal methylation by METTL11A/NTM1 is required for binding double-stranded DNA and stable chromatin association. Di- and trimethylation produce a permanent positive charge on the amino group, which facilitates electrostatic binding to the phosphate groups on DNA, while inhibiting histone-binding. Methylated tail helps retain RCC1 on chromosomes during nucleotide exchange on Ran.
Celluar localization	Nucleus;
UniPort	P18754