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Rabbit Anti-RBPJ Polyclonal Antibody#abs136965

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Description

Catalog-specification	Delivery time	USD price
abs136965-100ug	1-2 Weeks	301.0
abs136965-50ug	1-2 Weeks	201.0

Overview
catalog	abs136965
Description	This gene encodes a vital transcriptional regulator involved in the Notch signaling pathway. The protein it produces acts as a repressor when it is not bound to Notch proteins, but functions as an activator when bound to them. Its mechanism of action is believed to involve the recruitment of chromatin remodeling complexes that contain either histone deacetylase or histone acetylase proteins to genes within the Notch signaling pathway. Multiple transcript variants, each encoding different isoforms, have been discovered for this gene. Additionally, there are several pseudogenes of this gene located on chromosome 9.
Other names	CBF-1, also known as recombination signal binding protein for immunoglobulin kappa J region, plays a pivotal role in regulating gene expression and development in various organisms. This protein, encoded by the RBPJ gene, is a transcription factor that binds to DNA sequences involved in gene activation and suppression. It is also a key regulator of the Notch signaling pathway, which is essential for cell fate determination in various tissues. Additionally, CBF-1 has been shown to be involved in several disease states, including cancer and cardiovascular disease, as well as autoimmune disorders. It has also been found to play a role in the immune response, with mutations in the RBPJ gene leading to immunodeficiency and other immune-related disorders. Overall, the multifaceted nature of CBF-1 underscores its importance as a target for therapeutic interventions in a wide range of diseases. Understanding its regulatory mechanisms and interactions with other cellular components will allow researchers to develop novel strategies for the treatment of various disorders.
Source	Rabbit
Specificity	The total amount of RBPJ naturally occurring in a sample can be detected using the RBPJ Antibody. The antibody recognizes and binds to all forms of RBPJ that are present within the sample.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Xenopus;Rabbit;Pig;Dog;Sheep;Bovine;
Antigen	RBPJ
Application	IHC 1:50-1:200, ELISA (peptide) 1:20000-1:40000, WB 1:500-1:2000. The dilution ranges for immunohistochemistry (IHC) are 1:50-1:200, whereas for enzyme-linked immunosorbent assay (ELISA) using peptide as the target, the dilution ranges are 1:20000-1:40000. Lastly, for Western blot (WB), the dilution ranges from 1:500 to 1:2000.
Immunogen	A synthesized peptide derived from human RBPJ.
MW	55kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen) (PubMed:23303788).
Celluar localization	Nucleus;
UniPort	Q06330