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Rabbit Anti-RPAB1 Polyclonal Antibody#abs141088

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Description

Catalog-specification	Delivery time	USD price
abs141088-100ug	1-2 Weeks	301.0
abs141088-50ug	1-2 Weeks	201.0

Overview
catalog	abs141088
Other names	The DNA-directed RNA polymerase II is composed of various subunits including III subunit ABC1, III subunit RPABC1, 23 kDa polypeptide, polypeptide E, subunit E, hRPB25, hsRPB5, Polr2e, Polymerase (RNA) II (DNA directed) polypeptide E (25kD), Polymerase (RNA) II (DNA directed) polypeptide E. This enzyme plays a crucial role in transcribing DNA into RNA. It is involved in the synthesis of RNA molecules, particularly mRNA, which serves as a template for protein production. The RNA polymerases I and III subunit RPABC1 are also important components of this complex. Additionally, XAP4 and RPB5 homolog are associated with the DNA-directed RNA polymerase II. The presence of these subunits ensures the proper functioning and regulation of transcription processes mediated by RNA polymerases I, II, and III.
Source	Rabbit
Specificity	The detection of endogenous levels of total RPAB1 is achieved by using the RPAB1 Antibody.
Species Reactivity	Human;Mouse;Rat
Antigen	RPAB1
Application	The dilutions for western blot (WB) range from 1:500 to 1:1000, for immunofluorescence (IF) and immunocytochemistry (ICC) the dilutions range from 1:100 to 1:500, and for ELISA (peptide) the dilutions range from 1:20000 to 1:40000. Please note that the generated content should be completely different from the original text and not follow the conversation style of ChapGPT.
Immunogen	A synthesized peptide.
MW	25 KD
Properties
Concentration	1mg/ml
purification	The SulfoLink™ Coupling Resin played an instrumental role in purifying the antiserum via peptide affinity chromatography. This innovative technique allowed for the isolation of highly specific peptides, resulting in a purified antiserum product.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the integrity of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid any type of temperature fluctuations, especially repeated freeze/thaw cycles as they may affect the quality of the product. Take caution to prevent any damage to the container and ensure proper labeling for easy identification. Following these guidelines will help guarantee the stability and effectiveness of the product during its shelf life.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity).
Celluar localization	Cytosol;Nucleus;
UniPort	P19388