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Rabbit Anti-POFUT1 Polyclonal Antibody#abs141163

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Description

Catalog-specification	Delivery time	USD price
abs141163-100ug	1-2 Weeks	301.0
abs141163-50ug	1-2 Weeks	201.0

Overview
catalog	abs141163
Other names	GDP-fucose protein O-fucosyltransferase 1, also known as O Fuc T or O FucT1, belongs to the family of O fucosyltransferase proteins. This enzyme plays a crucial role in the post-translational modification of proteins, particularly in the addition of O-fucose glycans to specific serine or threonine residues of target proteins. Pofut 1, another name for Peptide-O-fucosyltransferase 1, is responsible for catalyzing this reaction. OFUT1_HUMAN is the gene that codes for this enzyme in humans. The proper functioning of POFUT1 is critical for the development and maintenance of various organs and tissues, including the brain and cardiovascular system. Overall, this enzyme is essential for normal physiological processes, and its dysfunction can lead to various diseases.
Source	Rabbit
Specificity	The antibody POFUT1 is capable of detecting the total endogenous levels of POFUT1 protein. By rearranging the provided content, it can be stated that the POFUT1 Antibody specifically recognizes and measures the levels of total POFUT1 protein present in an organism.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Chicken;Sheep;Dog;Horse;Bovine;
Antigen	POFUT1
Application	To obtain optimal results, we recommend using a dilution of WB 1:500~1:1000, IHC: 1:50~1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000. It is important to note that a dilution too low or too high may compromise the accuracy of your results. Therefore, it is essential to ensure that accurate dilution factors are employed at all times during sample preparation and experimentation. By following these guidelines, you can generate reliable and accurate data for your research needs.
Immunogen	A synthesized peptide.
MW	44 KD
Properties
Concentration	1mg/ml
purification	Using the SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. This process effectively removed impurities and isolated the desired components of the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	One year of storage at a temperature of -20 °C is recommended. To avoid damage, do not subject the item to repeated cycles of freezing and thawing. It is important to follow these guidelines to ensure the longevity and quality of the product.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue found in the consensus sequence C2-X(4,5)-[S/T]-C3 of EGF domains, where C2 and C3 are the second and third conserved cysteines. Specifically uses GDP-fucose as donor substrate and proper disulfide pairing of the substrate EGF domains is required for fucose transfer. Plays a crucial role in NOTCH signaling. Initial fucosylation of NOTCH by POFUT1 generates a substrate for FRINGE/RFNG, an acetylglucosaminyltransferase that can then extend the fucosylation on the NOTCH EGF repeats. This extended fucosylation is required for optimal ligand binding and canonical NOTCH signaling induced by DLL1 or JAGGED1. Fucosylates AGRN and determines its ability to cluster acetylcholine receptors (AChRs).
Tissue specificity	Highly expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Posttranslational modification	N-glycosylated.
Celluar localization	Endoplasmic reticulum;
UniPort	Q9H488