Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-HMGB1 Polyclonal Antibody#abs132003

Please note that the price listed above is for reference only. For specific pricing details, please contact our seller Vecent. It's important to understand that the price may vary depending on various factors, so it's recommended to consult with our seller for accurate pricing information. Thank...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs132003-50ug	1-2 Weeks	201.0
abs132003-100ug	1-2 Weeks	301.0

Overview
catalog	abs132003
Description	HMG proteins 1 and 2 are found throughout chromatin and are not associated with histones. Research indicates that when HMG proteins bind to DNA, they cause changes in the structure of the DNA, such as bending and unwinding of the helix. These proteins work together with Oct-2, members of the NFκB family, ATF-2, and c-Jun to activate the process of transcription. It is believed that their combined action leads to the activation of gene expression. Please note that the generated content is based on the original text information but rearranged to provide a different perspective.
Other names	The protein known as Amphoterin, or High Mobility Group Box 1 (HMGB1), is a nonhistone chromosomal protein that plays an essential role in many biological processes. It is also known as a sulfoglucuronyl carbohydrate binding protein or SBP1, and is encoded by the HMGB1 gene. HMGB1 is a versatile protein that can bind to DNA, RNA, and other proteins, and is involved in regulating gene expression, DNA repair, and many other cellular functions. It has also been implicated in various diseases, including cancer, inflammation, and autoimmune disorders. The HMGB1 protein is structurally composed of two DNA-binding domains, known as HMG boxes, connected by a negatively charged protein tail. This structural configuration allows it to interact with DNA and other molecules in different ways, depending on the cellular context. Research into HMGB1 is ongoing, and discoveries regarding its roles in various biological processes continue to emerge. It is considered a promising target for therapeutic interventions in a variety of diseases, and efforts to develop drugs that target HMGB1 are currently underway.
Source	Rabbit
Specificity	The HMGB1 antibody has the ability to identify total HMGB1 levels that are naturally occurring within the body. This information can be used to help analyze and understand various biological processes. By utilizing this antibody, researchers can gain valuable insights into the role that HMGB1 plays in disease and other physiological conditions.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Pig;Dog;Horse;Bovine
Antigen	HMGB1
Application	The recommended dilution for western blotting (WB) ranges from 1:500 to 1:2000, for immunohistochemistry (IHC) it is 1:200, for immunofluorescence/immunocytochemistry (IF/ICC) it is 1:100 to 1:500, and for ELISA with peptide-based assays it is 1:20000 to 1:40000. Feel free to ask for any further clarification regarding dilution recommendations.
Immunogen	A synthesized peptide derived from human HMGB1.
MW	24kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury (PubMed:27362237). Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance (PubMed:23519706, PubMed:23446148, PubMed:23994764, PubMed:25048472). Has proangiogdenic activity (By similarity). May be involved in platelet activation (By similarity). Binds to phosphatidylserine and phosphatidylethanolamide (By similarity). Bound to RAGE mediates signaling for neuronal outgrowth (By similarity). May play a role in accumulation of expanded polyglutamine (polyQ) proteins such as huntingtin (HTT) or TBP (PubMed:23303669, PubMed:25549101).
Tissue specificity	Ubiquituous. Expressed in platelets (PubMed:11154118).
Posttranslational modification	Phosphorylated at serine residues. Phosphorylation in both NLS regions is required for cytoplasmic translocation followed by secretion (PubMed:17114460).Acetylated on multiple sites upon stimulation with LPS (PubMed:22801494). Acetylation on lysine residues in the nuclear localization signals (NLS 1 and NLS 2) leads to cytoplasmic localization and subsequent secretion (By similarity). Acetylation on Lys-3 results in preferential binding to DNA ends and impairs DNA bending activity (By similarity).Reduction/oxidation of cysteine residues Cys-23, Cys-45 and Cys-106 and a possible intramolecular disulfide bond involving Cys-23 and Cys-45 give rise to different redox forms with specific functional activities in various cellular compartments: 1- fully reduced HMGB1 (HMGB1C23hC45hC106h), 2- disulfide HMGB1 (HMGB1C23-C45C106h) and 3- sulfonyl HMGB1 (HMGB1C23soC45soC106so).Poly-ADP-ribosylated by PARP1 when secreted following stimulation with LPS (By similarity).In vitro cleavage by CASP1 is liberating a HMG box 1-containing peptide which may mediate immunogenic activity; the peptide antagonizes apoptosis-induced immune tolerance (PubMed:24474694). Can be proteolytically cleaved by a thrombin:thrombomodulin complex; reduces binding to heparin and proinflammatory activities (By similarity).
Celluar localization	Endosome;Extracellular region or secreted;Nucleus;Plasma Membrane;
UniPort	P09429

This product is for research use only, not for use in diagnostic prodecures or in human.

Hot Tags: rabbit anti-hmgb1 polyclonal antibody#abs132003, China rabbit anti-hmgb1 polyclonal antibody#abs132003 suppliers

Previous:Rabbit Anti-Cleaved-caspase3 Polyclonal Antibody#abs132005

Next:Rabbit Anti-RAB11FIP1 Polyclonal Antibody#abs139315

Send Inquiry