Rabbit Anti-PARK7 Polyclonal Antibody #abs130311

abs130311-50ug

1-2 Weeks

201

abs130311-100ug

1-2 Weeks

301

Description

Autosomal recessive early onset parkinsonism has been linked to a protein called DJ-1. This protein plays a crucial role in the body's response to oxidative stress. When exposed to hydrogen peroxide, three specific cysteines in DJ-1 are known to become oxidized to cysteine sulphonic acid. Any loss of DJ-1 function can lead to neurodegeneration over time. It is essential to understand these mechanisms since they play an important role in Parkinson's disease and related conditions.

Other names

The protein known as DJ-1, also referred to as CAP1, has multiple aliases including DJ1, Epididymis secretory sperm binding protein Li 67p, FLJ27376, FLJ34360, FLJ92274, HEL S 67p, Oncogene DJ1, OTTHUMP00000001348, OTTHUMP00000001349, OTTHUMP00000001350, OTTHUMP00000001351, PARK7, PARK7_HUMAN, Parkinson disease (autosomal recessive, early onset) 7, Parkinson disease protein 7, Parkinson protein 7, Protein DJ-1, and SP22. This protein is involved in various cellular processes and is of particular interest in relation to Parkinson's disease. By rearranging the provided information, a highly similar content can be generated while ensuring the preservation of the original text details.

Source

Rabbit

Specificity

The detection of total PARK7 using the PARK7 antibody is capable of identifying endogenous levels of the protein. Please rearrange the content to generate a highly similar statement.

Species Reactivity

Human;Mouse;Rat

Application

WB: 1:500~1:3000, ELISA(peptide) 1:20000-1:40000

Immunogen

A synthesized peptide derived from human PARK7

Properties

Concentration

1mg/ml

Purification

Affinity-chromatography

Clonality

Polyclonal Antibody

Stability & Storage

To preserve the quality of your product, it is important to store it at a temperature of -20 °C for a period of one year. It is also recommended that you avoid subjecting the product to repeated cycles of freezing and thawing. By following these guidelines, you can ensure that your product remains stable and effective for its intended use.

Storage buffer

The recommended storage conditions for Rabbit IgG are at a temperature of -20 °C. The solution should be prepared in phosphate buffered saline with a pH of 7.4, containing 150mM NaCl and 0.02% sodium azide. Additionally, 50% glycerol should be added to the solution. It is important to note that the stability of the solution is guaranteed for a period of 12 months from the date of receipt.

Target

Background

This protein is an important enzyme that plays a role in repairing damaged proteins and nucleotides. It specifically targets the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. By catalyzing the deglycation process, it removes these adducts and restores the integrity of the proteins and nucleotides.
Not only does it repair glycated proteins, but it also releases lactate or glycolate as byproducts. The protein deglycase activity of this enzyme is particularly effective in reversing glycation of cysteine, arginine, and lysine residues in proteins. This reactivation process helps prevent the formation of advanced glycation endproducts (AGE), which can cause irreversible damage.
Additionally, this enzyme acts as a nucleotide deglycase, repairing glycated guanine in both free nucleotides and in DNA and RNA molecules. This function is part of a nucleotide repair system called the guanine glycation repair (GG repair) system, which aims to reverse the damage caused by methylglyoxal and glyoxal in nucleotides.
The enzyme also exhibits glyoxalase activity, although it is primarily a reflection of its deglycase activity. Its important role in cell protection against oxidative stress and cell death is attributed to its function as an oxidative stress sensor and a redox-sensitive chaperone and protease. It plays a crucial role in neuroprotection, male fertility, cell growth, and transformation, as well as in regulating various signaling pathways.
Furthermore, this enzyme eliminates hydrogen peroxide and protects cells from hydrogen peroxide-induced cell death. It is involved in maintaining correct mitochondrial morphology and function and in the autophagy of dysfunctional mitochondria. It also regulates the expression and stability of certain mitochondrial uncoupling proteins, protecting cells from oxidative stress.
In astrocytes and neuronal cells, the enzyme modulates inflammatory responses and lipid rafts-dependent endocytosis. In pancreatic islets, it helps maintain mitochondrial reactive oxygen species (ROS) levels and glucose homeostasis. It protects pancreatic beta cells from cell death caused by inflammation and cytotoxicity.
The enzyme also has the ability to bind copper as well as toxic mercury ions, contributing to the cell's defense mechanism against metal toxicity. In macrophages, it interacts with a specific subunit to direct NADPH oxidase-dependent ROS production and provides protection against sepsis.
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Tissue specificity

Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain (at protein level). Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa. Expressed by pancreatic islets at higher levels than surrounding exocrine tissues (PubMed:22611253).

Posttranslational modification

Sumoylated on Lys-130 by PIAS2 or PIAS4; which is enhanced after ultraviolet irradiation and essential for cell-growth promoting activity and transforming activity.Cys-106 is easily oxidized to sulfinic acid.Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress.

Celluar localization

Cytosol;Endoplasmic reticulum;Extracellular region or secreted;Mitochondrion;Nucleus;Plasma Membrane;

UniPort

Q99497