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Rabbit Anti-NSMCE2 Polyclonal Antibody#abs138995

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Description

Catalog-specification	Delivery time	USD price
abs138995-100ug	1-2 Weeks	301.0
abs138995-50ug	1-2 Weeks	201.0

Overview
catalog	abs138995
Other names	The C8orf36 gene, also known as Non-SMC element 2 homolog (NSE2), codes for a protein called E3 SUMO-protein ligase NSE2 or hMMS21. This protein is crucial for maintaining the integrity of chromosomes and is also involved in DNA repair mechanisms. It is also referred to as MMS21 homolog or methyl methanesulfonate sensitivity gene 21. The NSE2 protein is a vital component of the non-SMC element 2 complex, which plays a significant role in regulating DNA architecture and chromosome condensation during cell division. Mutations in the NSE2 gene have been linked to various genetic disorders, including chromosomal instability and developmental defects. Research has shown that NSE2 acts as a SUMO ligase, which means that it is responsible for attaching small ubiquitin-like modifier (SUMO) proteins to target substrates. This process, known as SUMOylation, plays a crucial role in regulating various cellular processes, including transcription, DNA repair, and chromosome segregation. In conclusion, the NSE2 protein plays a critical role in regulating DNA architecture and chromosome condensation. It acts as a SUMO ligase and helps ensure the proper functioning of various cellular processes. Mutations in the NSE2 gene have been linked to several genetic disorders, highlighting the protein's importance in maintaining chromosomal integrity.
Source	Rabbit
Specificity	The NSMCE2 Antibody is designed to identify total NSMCE2 levels within the body. Its function is to detect these levels in an endogenous manner.
Species Reactivity	Human
Antigen	NSMCE2
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human NSMCE2.
MW	28 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. To produce a similar content, the sentence structure has been rearranged while retaining the original information.
Clonality	Polyclonal Antibody
Stability & Storage	To preserve the quality of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid any instances of repeat freezing and thawing cycles as this may have negative effects on the product's integrity and efficacy. Therefore, it is crucial to take the necessary precautions to ensure that the product is stored in optimal conditions.
Storage buffer	This product contains Rabbit IgG in a phosphate buffered solution with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C and is stable for a period of 12 months from the date of receipt.
Target
Background	E3 SUMO-protein ligase component of the SMC5-SMC6 complex, a complex involved in DNA double-strand break repair by homologous recombination. Is not be required for the stability of the complex. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs). Acts as an E3 ligase mediating SUMO attachment to various proteins such as SMC6L1 and TRAX, the shelterin complex subunits TERF1, TERF2, TINF2 and TERF2IP, and maybe the cohesin components RAD21 and STAG2. Required for recruitment of telomeres to PML nuclear bodies. SUMO protein-ligase activity is required for the prevention of DNA damage-induced apoptosis by facilitating DNA repair, and for formation of APBs in ALT cell lines. Required for sister chromatid cohesion during prometaphase and mitotic progression.
Posttranslational modification	Sumoylated, possibly via autosumoylation.
Celluar localization	Nucleus;
UniPort	Q96MF7