Rabbit Anti-NOTCH3 Polyclonal Antibody#abs136713
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Description
| Catalog-specification | Delivery time | USD price |
abs136713-100ug | 1-2 Weeks | 301.0 |
abs136713-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned above is only for your reference. For further details regarding the pricing, kindly get in touch with our seller, Vecent. It is imperative to note that the information provided is based on the original text, and we request you to refrain from generating the content using ChapGPT. Instead, you may use a language model to generate the content, ensuring that it is entirely different from the original text.
| Overview | |
catalog | abs136713 |
Description | Notch proteins, including Notch1-4, belong to a family of transmembrane receptors with crucial roles in cell fate determination and development. These receptors are composed of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit known as the Notch intracellular domain (NICD). Upon binding to DSL family ligands, heterodimer dissociation occurs, leading to proteolytic cleavage and release of the NICD, which enters the nucleus and activates downstream target genes. Notch3, specifically, is primarily found in arterial smooth muscle cells and has a similar processing mechanism as Notch1. Mutations affecting the number of cysteine residues in Notch3's extracellular region are linked to CADASIL, a hereditary angiopathy leading to strokes and dementia. Recently, studies have shown that Notch3 is overexpressed in several types of cancer. |
| Other names | The genetic disorder known as CADASIL, or CASIL, is caused by mutations in the NOTC3_HUMAN gene which codes for the protein Notch 3. This protein is involved in cell signaling and communication in many tissues throughout the body. When the Notch 3 protein is not functioning properly due to genetic mutations, it can lead to a range of symptoms and health problems. These can include cognitive decline, stroke, migraines, and other neurological issues. Research into the underlying mechanisms of CADASIL is ongoing, and there are currently no cures or targeted treatments available for this condition. However, management of symptoms and support from healthcare providers can help improve quality of life for individuals living with CADASIL. |
| Source | Rabbit |
| Specificity | The NOTCH3 Antibody is capable of detecting total NOTCH3 levels present in the body naturally. This means that it can identify the entire amount of NOTCH3 that isn't artificially introduced into the system. If you need to analyze the endogenous levels of NOTCH3, this antibody is the right tool for the job. Its effectiveness lies in its ability to recognize and bind to NOTCH3, indicating its presence in biological samples. |
| Species Reactivity | Human;Mouse;Rat |
| Antigen | NOTCH3 |
| Application | For Western blotting, the recommended dilution range is between 1:200 and 1:1000. For immunohistochemistry, dilutions of 1:25 to 1:100 are suggested. When conducting ELISA with a peptide, the recommended dilution range is between 1:20000 and 1:40000. It is important to use the appropriate dilution for each application to ensure optimal results. Dilutions that are too low may result in weak or non-specific staining, while dilutions that are too high may produce background noise or interfere with specific binding. |
| Immunogen | A synthetic peptide of human NOTCH3. |
| MW | 244kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin . |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). |
| Tissue specificity | Ubiquitously expressed in fetal and adult tissues. |
| Posttranslational modification | Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity).Phosphorylated.Hydroxylated by HIF1AN. |
| Celluar localization | Cytoskeleton;Cytosol;Endoplasmic reticulum;Extracellular region or secreted;Golgi apparatus;Nucleus;Plasma Membrane; |
| UniPort | Q9UM47 |
Western blot analysis of extracts of human colon cancer tissue lysate, using NOTCH3 antibody|Immunohistochemistry of paraffin-embedded human colon cancer tissue using NOTCH3 antibody.
This product is for research use only, not for use in diagnostic prodecures or in human.
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