Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-FLT3 Polyclonal Antibody#abs138066

Please note that the price stated above is only for reference purposes. For the detailed pricing, kindly get in touch with our seller Vecent. Also, please be informed that the content generated from ChapGPT will not be used for the conversation. Instead, our language model will be utilized to...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs138066-100ug	1-2 Weeks	301.0
abs138066-50ug	1-2 Weeks	201.0

Overview
catalog	abs138066
Other names	The CD135 antigen, also known as FLT3 (Fms-like tyrosine kinase 3) or FL cytokine receptor, is a growth factor receptor tyrosine kinase type III. It is also referred to as fetal liver kinase 2 (Flk 2), Ly-72, or stem cell tyrosine kinase 1 (Stk 1). The protein encoded by the FLT3 gene plays a crucial role in the regulation of cell growth and differentiation. It is a member of the FMS-like tyrosine kinase receptor family and is predominantly found on hematopoietic stem and progenitor cells. The FLT3 receptor is known to be involved in the development and maintenance of the hematopoietic system. Its activation by its ligand, FL (Fms-like tyrosine kinase ligand), leads to the activation of downstream signaling pathways that regulate cell proliferation, survival, and differentiation. Abnormalities in FLT3, such as mutations or overexpression, have been associated with various hematological malignancies, including acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). Moreover, the FLT3 receptor has gained significant attention as a therapeutic target in the treatment of these diseases. Various FLT3 inhibitors have been developed and tested in clinical trials, showing promising results in targeting and inhibiting the aberrant activity of FLT3 in cancer cells. In summary, FLT3 is a crucial receptor kinase involved in the regulation of hematopoiesis, and its dysregulation is associated with malignancies. Understanding the functions and dysregulation of FLT3 provides valuable insights into the development of targeted therapies for hematological disorders.
Source	Rabbit
Specificity	The total FLT3 present in the body can be detected by using the FLT3 Antibody. This antibody is capable of identifying the endogenous levels of FLT3. To create similar content using the same information, you could say: The endogenous levels of total FLT3 can be detected using the FLT3 Antibody. This antibody accurately identifies the presence of FLT3 in the body, making it an effective tool for studying FLT3 and its functions.
Species Reactivity	Human;Mouse;Rat
Antigen	FLT3
Application	IHC 1:200, IF/ICC 1:100-1:500, ELISA (peptide) 1:20000-1:40000, WB 1:1000-3000. These are the recommended dilutions for various assays. For Western blotting (WB), a dilution range of 1:1000-3000 is commonly used. For immunohistochemistry (IHC), a dilution of 1:200 is recommended. For immunofluorescence/immunocytochemistry (IF/ICC), the optimal dilution ranges from 1:100 to 1:500. Lastly, for enzyme-linked immunosorbent assay (ELISA) with peptide antigens, the suggested dilution is 1:20000-1:40000. It is important to adjust the dilution according to the specific experiment and the reactivity of the antibody.
Immunogen	A synthesized peptide derived from human FLT3.
MW	112 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed in the purification of the antiserum through peptide affinity chromatography. Let me create a rephrased version of the given information while maintaining its original context.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage conditions, it is recommended to keep the product at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing, as this can compromise its quality and effectiveness. Taking these precautions can help maintain the integrity of the product and ensure its long-term stability.
Storage buffer	Store at -20 °C, the Rabbit IgG is suspended in phosphate buffered saline with a pH of 7.4. The solution contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is important to note that this product is stable for a period of 12 months from the date of receipt.
Target
Background	Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.
Tissue specificity	Detected in bone marrow, in hematopoietic stem cells, in myeloid progenitor cells and in granulocyte/macrophage progenitor cells (at protein level). Detected in bone marrow, liver, thymus, spleen and lymph node, and at low levels in kidney and pancreas. Highly expressed in T-cell leukemia.
Posttranslational modification	N-glycosylated, contains complex N-glycans with sialic acid.Autophosphorylated on several tyrosine residues in response to FLT3LG binding. FLT3LG binding also increases phosphorylation of mutant kinases that are constitutively activated. Dephosphorylated by PTPRJ/DEP-1, PTPN1, PTPN6/SHP-1, and to a lesser degree by PTPN12. Dephosphorylation is important for export from the endoplasmic reticulum and location at the cell membrane.Rapidly ubiquitinated by UBE2L6 and the E3 ubiquitin-protein ligase SIAH1 after autophosphorylation, leading to its proteasomal degradation.
Celluar localization	Cytosol;Endoplasmic reticulum;Nucleus;Plasma Membrane;
UniPort	P36888