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Rabbit Anti-Nidogen Polyclonal Antibody#abs140485

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Description

Catalog-specification	Delivery time	USD price
abs140485-100ug	1-2 Weeks	301.0
abs140485-50ug	1-2 Weeks	201.0

Overview
catalog	abs140485
Other names	Nidogen, also known as Enactin, Ent, Entactin 1, or NID 1, is a structural protein present in various tissues of the human body. Its primary function is to provide support and stability to the extracellular matrix by binding to other matrix proteins, such as laminin and collagen. Nidogen plays a vital role in tissue development and differentiation, as well as in maintaining tissue integrity. The protein is encoded by the NID1 gene, also referred to as NID or NID-1. In humans, two isoforms of nidogen exist, namely nidogen-1 and nidogen-2. Nidogen-1 is primarily found in basement membranes of various tissues, whereas nidogen-2 is mostly present in skeletal muscle and peripheral nerve. The Nid1 gene is conserved across different species, indicating its evolutionary significance. Nidogen is implicated in various pathological conditions, including cancer, inflammation, and neurodegeneration. Alterations in nidogen expression have been reported in several types of cancer, including breast and prostate cancer. The protein has also been shown to modulate immune cell function and contribute to inflammatory responses. Additionally, reduced levels of nidogen have been associated with neurodegenerative disorders such as Alzheimer's disease. In summary, nidogen is a critical extracellular matrix protein involved in tissue development, organization, and maintenance. Its dysregulation has been linked to various pathological conditions, highlighting its potential as a therapeutic target for various diseases.
Source	Rabbit
Specificity	The Nidogen Antibody is designed to identify the presence of total Nidogen at its natural levels within the body. The antibody recognizes and binds to endogenous Nidogen, allowing for its detection in various biological samples. By targeting total Nidogen, researchers can gain insights into its role and functions in different cellular processes. This antibody provides a valuable tool for studying and understanding Nidogen's contribution to physiological and pathological conditions.
Species Reactivity	Human
Predictive reaction species	Sheep;Bovine;Horse;Pig;
Antigen	Nidogen
Application	The recommended dilutions for various applications of this protein are as follows: For Western blotting, a dilution of 1:1000 is recommended. For IF/ICC, the recommended dilution range is 1:100 to 1:500. For ELISA using peptide as the antigen, the recommended dilution range is 1:20000 to 1:40000. These dilution ratios are critical for obtaining accurate and reliable results. It is important to adhere to the recommended dilutions to ensure the validity of experimental data.
Immunogen	A synthesized peptide derived from human Nidogen.
MW	136kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. A highly specific procedure, it helped to isolate the desired components and remove any unwanted impurities. The resulting product was of high quality, ready for use in a range of applications. Overall, this process offered an efficient and effective way to obtain pure antiserum for research or therapeutic purposes.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage conditions, it is recommended to keep the product at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this could negatively affect its quality and integrity. By following these storage guidelines, you can help ensure that the product remains stable and effective for its intended use.
Storage buffer	The Rabbit IgG is stored at -20 °C in a solution of phosphate buffered saline at pH 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It remains stable for 12 months from the date of receipt.
Target
Background	Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
Posttranslational modification	N- and O-glycosylated.
Celluar localization	Extracellular region or secreted;
UniPort	P14543