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Phospho-PRK1/2 (Thr774/816) Rabbit Polyclonal Antibody#abs140178

Phospho-PRK1/2 (Thr774/816) Rabbit Polyclonal Antibody#abs140178

Please be advised that the price provided is for your reference only. For detailed pricing information, kindly reach out to our seller, Vecent. Feel free to contact Vecent for further information. Western blot analysis PRK1/2 (Phospho-Thr774/816) using Na2VO3 treated HepG2 whole cell lysates...

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Description

Catalog-specificationDelivery timeUSD price

abs140178-100ug

1-2 Weeks

301.0

abs140178-50ug

1-2 Weeks

201.0

Please be advised that the price provided is for your reference only. For detailed pricing information, kindly reach out to our seller, Vecent. Feel free to contact Vecent for further information.


Overview

catalog

abs140178
Other namesProtease-activated kinase 1, also known as PAK1 or PKN alpha, is a serine-threonine protein kinase that belongs to the protein kinase C (PKC) family. It is also referred to as protein kinase N1 and is involved in various cellular processes, including cell survival, proliferation, and migration.
Protein kinase C-like 1 (PKC1) is another name for this enzyme, indicating its structural similarities to PKC. PKN1 is its official name, and it has also been referred to as PRK1 and PRKCL1.
PKN alpha is a critical player in several signaling pathways, including those initiated by growth factors and G-protein-coupled receptors. Its activity is regulated by phosphorylation, and it is activated via the interaction with the lipid cardiolipin. PKN alpha has been implicated in several pathologies, including cancer and Alzheimer's disease.
Protease-activated kinase 2 (PAK2) is another member of the PAK family of enzymes. It is also known as Cardiolipin activated protein kinase and has been shown to play a role in insulin signaling and cell migration.
PKN gamma or PKN2 is a third member of this family of serine-threonine kinases. It is also referred to as PRK2 and PRKCL2. Like PAK1 and PAK2, PKN2 is involved in multiple cellular processes, including cytoskeletal rearrangement and cell migration.
In summary, the PAK family of proteins, including PAK1, PAK2, and PKN alpha and gamma, are serine-threonine kinases that play critical roles in various cellular processes. These enzymes have been implicated in a wide range of pathological conditions and are, therefore, an attractive target for therapeutic intervention.
SourceRabbit
SpecificityThe Phospho-Thr774/816 Antibody, also known as PRK1/2 Antibody, is designed to specifically target and detect the phosphorylation of PRK1/2 at Thr774/816 residues. It enables the identification of endogenous levels of PRK1/2 protein only when it is phosphorylated at Thr774/816. By utilizing this antibody, researchers can gain valuable insights into the activation status and signaling pathways mediated by PRK1/2. Please note that this generated content has been based on the original text information and rearranged to convey a similar meaning in a different manner.
Species ReactivityHuman
Predictive reaction speciesSheep;Bovine;Xenopus;Pig;
AntigenPRK1/2
ApplicationWB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
ImmunogenA peptide that has been synthesized from human PRK1/2 and contains Phospho-Thr774/816 has been developed. The new content has been produced by rearranging the information from the original text, while maintaining a high level of similarity with the original content. It is important to note that the text has been generated using a language model, which has resulted in a very different output compared to the original text.
MW104KD
Properties

Concentration

1mg/ml

purificationThe purified rabbit serum yielded the antibody through sequential chromatography on affinity columns. These columns were specific for both phospho- and non-phospho-peptides, enabling purification by affinity. The resulting antibody is highly pure and similar in composition to the original serum.
ClonalityPolyclonal Antibody
Stability & StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage bufferRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

Target

Background

PKC-related serine/threonine-protein kinase involved in various processes such as regulation of the intermediate filaments of the actin cytoskeleton, cell migration, tumor cell invasion and transcription regulation. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Regulates the cytoskeletal network by phosphorylating proteins such as VIM and neurofilament proteins NEFH, NEFL and NEFM, leading to inhibit their polymerization. Phosphorylates 'Ser-575', 'Ser-637' and 'Ser-669' of MAPT/Tau, lowering its ability to bind to microtubules, resulting in disruption of tubulin assembly. Acts as a key coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-11' of histone H3 (H3T11ph), a specific tag for epigenetic transcriptional activation that promotes demethylation of histone H3 'Lys-9' (H3K9me) by KDM4C/JMJD2C. Phosphorylates HDAC5, HDAC7 and HDAC9, leading to impair their import in the nucleus. Phosphorylates 'Thr-38' of PPP1R14A, 'Ser-159', 'Ser-163' and 'Ser-170' of MARCKS, and GFAP. Able to phosphorylate RPS6 in vitro.

Tissue specificityFound ubiquitously. Expressed in heart, brain, placenta, lung, skeletal muscle, kidney and pancreas. Expressed in numerous tumor cell lines, especially in breast tumor cells.
Posttranslational modificationAutophosphorylated; preferably on serine. Phosphorylated during mitosis.Activated by limited proteolysis with trypsin.(Microbial infection) In case of infection, polyubiquitinated by the bacterial E3 ubiquitin-protein ligase SspH1, leading to its proteasomal degradation.
Celluar localizationCytosol;Endosome;Nucleus;Plasma Membrane;
UniPortQ16512/Q16513


Western blot analysis PRK1/2 (Phospho-Thr774/816) using Na2VO3 treated HepG2 whole cell lysates


This product is for research use only, not for use in diagnostic prodecures or in human.


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