Phospho-TIF1-beta (Ser824) Rabbit Polyclonal Antibody#abs140181
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Description
| Catalog-specification | Delivery time | USD price |
abs140181-100ug | 1-2 Weeks | 301.0 |
abs140181-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned is only for your reference and for detailed pricing information, it is recommended that you get in touch with our sales representative, Vecent. Kindly generate a unique and distinct content by rearranging the given information, based on the original text. Please avoid using the ChapGPT response generation method and instead use a language model to generate text in a completely different way.
| Overview | |
catalog | abs140181 |
| Other names | TRIM28 is a protein that functions as an E3 SUMO-protein ligase. Also known as KAP-1, KRAB-associated protein 1, or TRIM28, it is involved in a variety of cellular processes, including transcriptional regulation, DNA damage repair, and chromatin remodeling. This protein is essential for the maintenance of cellular homeostasis and is often dysregulated in cancer. TRIM28 interacts with a diverse range of binding partners, including transcription factors, epigenetic regulators, and DNA repair proteins. It is particularly known for its role as a co-repressor of transcription, recruiting histone deacetylases and other chromatin-modifying enzymes to silence gene expression. In addition to its transcriptional regulatory roles, TRIM28 is also involved in the DNA damage response. It interacts with various DNA repair proteins to facilitate the repair of damaged DNA and maintain genomic stability. Overall, TRIM28 is a versatile and important protein with crucial roles in many cellular processes. Dysregulation of TRIM28 function can have profound effects on cellular homeostasis and may contribute to the development of a variety of diseases, including cancer. |
| Source | Rabbit |
| Specificity | The TIF1-β (Phospho-Ser824) Antibody specifically identifies TIF1-β protein but solely when it is phosphorylated at Ser824, allowing for the detection of its natural expression levels. In order to create a highly similar content, the original information will be rearranged and represented differently. |
| Species Reactivity | Human;Mouse;Rat |
| Antigen | TIF1-β |
| Application | WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000 |
| Immunogen | TIF1-β（824）？，。 |
| MW | 88kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | Our rabbit serum-based antibody has undergone a rigorous purification process via sequential chromatography on both phospho- and non-phospho-peptide affinity columns. This process ensures that the resulting antibody is of the highest quality and similarity to the original protein. We are proud to offer a product that meets the high standards of our customers. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | To ensure proper storage, maintain a temperature of -20 °C for a duration of one year. It is crucial to avoid subjecting the product to multiple freeze/thaw cycles. |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes. Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. Recruitment of SETDB1 induces heterochromatinization. May play a role as a coactivator for CEBPB and NR3C1 in the transcriptional activation of ORM1. Also corepressor for ERBB4. Inhibits E2F1 activity by stimulating E2F1-HDAC1 complex formation and inhibiting E2F1 acetylation. May serve as a partial backup to prevent E2F1-mediated apoptosis in the absence of RB1. Important regulator of CDKN1A/p21(CIP1). Has E3 SUMO-protein ligase activity toward itself via its PHD-type zinc finger. Also specifically sumoylates IRF7, thereby inhibiting its transactivation activity. Ubiquitinates p53/TP53 leading to its proteosomal degradation; the function is enhanced by MAGEC2 and MAGEA2, and possibly MAGEA3 and MAGEA6. Mediates the nuclear localization of KOX1, ZNF268 and ZNF300 transcription factors. In association with isoform 2 of ZFP90, is required for the transcriptional repressor activity of FOXP3 and the suppressive function of regulatory T-cells (Treg) (PubMed:23543754). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610). Acts as a corepressor for ZFP568 (By similarity). |
| Tissue specificity | Expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. |
| Posttranslational modification | ATM-induced phosphorylation on Ser-824 represses sumoylation leading to the de-repression of expression of a subset of genes involved in cell cycle control and apoptosis in response to genotoxic stress. Dephosphorylation by the phosphatases, PPP1CA and PP1CB forms, allows sumoylation and expression of TRIM28 target genes.Sumoylation/desumoylation events regulate TRIM28-mediated transcriptional repression. Sumoylation is required for interaction with CHD3 and SETDB1 and the corepressor activity. Represses and is repressed by Ser-824 phosphorylation. Enhances the TRIM28 corepressor activity, inhibiting transcriptional activity of a number of genes including GADD45A and CDKN1A/p21. Lys-554, Lys-779 and Lys-804 are the major sites of sumoylation. In response to Dox-induced DNA damage, enhanced phosphorylation on Ser-824 prevents sumoylation and allows de-repression of CDKN1A/p21.Auto-ubiquitinated; enhanced by MAGEA2 and MAGEC2.Citrullinated by PADI4. |
| Celluar localization | Nucleus; |
| UniPort | Q13263 |
Western blot analysis TIF1-β (Phospho-Ser824) using serum treated 293 whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
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