Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-MFGE8 Polyclonal Antibody#abs139704

Please note that the above price is for reference only. For detailed pricing information, kindly reach out to our seller Vecent. It is important to highlight that the content generated using language models will be significantly different from the text produced by ChapGPT. Therefore, kindly...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs139704-100ug	1-2 Weeks	301.0
abs139704-50ug	1-2 Weeks	201.0

Overview
catalog	abs139704
Other names	Antigen BA46, also known as Breast epithelial antigen BA46, is a protein with various aliases including EDIL1, HMFG, hP47, HsT19888, Lactadherin, Medin, MFG-E8, MFGE8, MFGM, MFGM_HUMAN, Milk fat globule EGF factor 8, Milk fat globule EGF factor 8 protein, Milk fat globule-EGF factor 8, O acetyl disialoganglioside synthase, OAcGD3S, SED1, SPAG10, and Sperm associated antigen 10. This protein, known as hP47 on the surface of sperm, plays a crucial role in various biological processes. The identification of BA46 in breast epithelial cells raises interest in its potential involvement in breast cancer development and progression. BA46, also referred to as Lactadherin, functions as a bridging molecule between apoptotic cells and phagocytes. It is involved in the clearance of apoptotic cells, a process known as efferocytosis, which is essential for tissue homeostasis and inflammation resolution. Additionally, BA46 has been found to possess other functions. It plays a role in mammary gland development and lactation by facilitating the formation of milk fat globules. BA46 is also implicated in regulating immune responses by modulating T-cell activity and promoting the release of anti-inflammatory factors. Furthermore, studies have indicated that BA46 may have implications beyond breast tissue. It has been observed in other tissues, such as the reproductive system, where it is present on the surface of sperm. This suggests its involvement in sperm function, particularly in sperm-egg interactions during fertilization. In conclusion, BA46 is a multifunctional protein with diverse roles in breast epithelial cells, apoptotic cell clearance, mammary gland development, immune modulation, and sperm physiology. Further research is necessary to fully understand the molecular mechanisms underlying the functions of this protein and its potential implications in various physiological and pathological processes.
Source	Rabbit
Specificity	The MFGE8 Antibody is capable of detecting total levels of endogenous MFGE8. This information can be rearranged to create the following statement: Total endogenous levels of MFGE8 can be detected using the MFGE8 Antibody.
Species Reactivity	Human;Mouse
Antigen	MFGE8
Application	1:1000-3000, WB, 1:200, IHC, 1:20000-1:40000, ELISA (peptide). The above-mentioned content provides information about the dilutions used for different laboratory techniques. For Western Blot (WB), the dilution range is 1:1000-3000. Immunohistochemistry (IHC) requires a dilution of 1:200. In the case of ELISA (peptide), the dilution range is relatively higher at 1:20000-1:40000. These dilution ratios are crucial for obtaining accurate and reliable results in various experiments and assays.
Immunogen	A synthesized peptide derived from human MFGE8.
MW	43 kDa
Properties
Concentration	1mg/ml
purification	The purification process of the antiserum involved the use of SulfoLink™ Coupling Resin for peptide affinity chromatography. This methodology enables a highly effective and specific purification of the desired antibodies. By following this approach, we were able to obtain highly purified antiserum with optimal quality.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure preservation, it is recommended to store the item at a temperature of -20 °C for a duration of one year. It is important to avoid subjecting it to repetitive cycles of freezing and thawing.
Storage buffer	The rabbit IgG is stored in a solution of phosphate buffered saline at a pH of 7.4. It contains 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C. This product is stable for a period of 12 months from the date of receipt.
Target
Background	Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction. Binds specifically to rotavirus and inhibits its replication.
Tissue specificity	Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.
Posttranslational modification	Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.
Celluar localization	Endoplasmic reticulum;Extracellular region or secreted;Plasma Membrane;
UniPort	Q08431