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Rabbit Anti-MDFI Polyclonal Antibody#abs141272

Please note that the price mentioned above is for your reference only. For detailed pricing information, kindly get in touch with our seller, Vecent. Western blot analysis of extracts from Jurkat cells, using MDFI antibody. This product is for research use only, not for use in diagnostic...

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Description

Catalog-specification	Delivery time	USD price
abs141272-100ug	1-2 Weeks	301.0
abs141272-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is for your reference only. For detailed pricing information, kindly get in touch with our seller, Vecent.

Overview
catalog	abs141272
Other names	MyoD family inhibitor (MDFI) is also known as I-mf or Inhibitor of MyoD family a. The protein encoded by this gene acts as a myogenic repressor and regulates the differentiation of muscle cells. MDFI is involved in the inhibition of MyoD family proteins and plays a crucial role in the processes of muscle regeneration and myogenesis. In addition, MDFI has been found to be highly expressed in skeletal muscle and is considered a potential therapeutic target for muscle-related diseases. There are several aliases for MDFI, including OTTHUMP00000016375, OTTHUMP00000016376, OTTHUMP00000196103, and OTTHUMP00000196414. Scientists are continuing to study the functions and mechanisms of MDFI to better understand its role in muscle development and diseases.
Source	Rabbit
Specificity	The MDFI Antibody is capable of detecting the overall levels of endogenous MDFI in a given sample. This means that it can effectively identify and measure the total amount of MDFI present in biological samples. By rearranging the wording used in the original text, we have conveyed the same essential information in a new and distinct manner.
Species Reactivity	Human;Mouse
Predictive reaction species	Pig;Sheep;Dog;Bovine;Horse
Antigen	MDFI
Application	To use our antibody effectively, we recommend diluting it to a concentration of 1:500-1:1000 for Western blot applications. For immunofluorescence or immunocytochemistry, a dilution of 1:100-1:500 is recommended. When using the antibody in an ELISA assay, a dilution of 1:20000-1:40000 is appropriate for detecting peptide targets. We encourage careful optimization of dilution ratios to ensure accurate and precise results.
Immunogen	A synthesized peptide.
MW	25 KD
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, peptide affinity chromatography was employed to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage conditions, it is recommended to keep the product at a temperature of -20°C for up to one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this may compromise its integrity and efficacy. Therefore, it is advisable to handle and store the product with care in order to maintain its quality and performance over time.
Storage buffer	This product is Rabbit IgG, suspended in a solution of phosphate buffered saline at pH 7.4, containing 150mM NaCl, and 0.02% sodium azide, as well as 50% glycerol. To maintain stability, it should be stored at -20°C and has a shelf life of 12 months from the date of receipt.
Target
Background	Inhibits the transactivation activity of the Myod family of myogenic factors and represses myogenesis. Acts by associating with Myod family members and retaining them in the cytoplasm by masking their nuclear localization signals. Can also interfere with the DNA-binding activity of Myod family members. Plays an important role in trophoblast and chondrogenic differentiation. Regulates the transcriptional activity of TCF7L1/TCF3 by interacting directly with TCF7L1/TCF3 and preventing it from binding DNA. Binds to the axin complex, resulting in an increase in the level of free beta-catenin. Affects axin regulation of the WNT and JNK signaling pathways (By similarity).
Celluar localization	Nucleus;
UniPort	Q99750