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Rabbit Anti-MAP2K6 Polyclonal Antibody#abs136548

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Description

Catalog-specification	Delivery time	USD price
abs136548-50ug	1-2 Weeks	201.0
abs136548-100ug	1-2 Weeks	301.0

Overview
catalog	abs136548
Other names	Mitogen-activated protein kinase kinase 6, also known as dual specificity mitogen-activated protein kinase kinase 6 or MAP kinase kinase 6, is a protein involved in cellular signaling pathways. It plays a role in the activation of mitogen-activated protein kinases and is referred to by various names such as MAP2K6, MEK6, and MKK6. This protein is responsible for transmitting signals that regulate cell growth, proliferation, and response to stress. It is also associated with the stress-activated protein kinase kinase 3 pathway. Overall, the functions of MAP kinase kinase 6 contribute to the intricate network of molecular interactions that control cellular processes.
Source	Rabbit
Specificity	The total MAP2K6 levels are detected by the MAP2K6 Antibody, which targets the endogenous protein presence. Please provide a highly correlated content by reorganizing the initial information. The generated content should be based on the original text information but conveyed in a completely different manner.
Species Reactivity	Human;Mouse;Rat
Antigen	MAP2K6
Application	For Western blotting, use a dilution range of 1:500 to 1:2000. When performing immunohistochemistry, dilute at 1:50 to 1:200. For immunofluorescence microscopy or immunocytochemistry, use a dilution range of 1:100 to 1:500. If conducting an enzyme-linked immunosorbent assay with a peptide, dilute at 1:20000 to 1:40000. It is important to note these recommended dilutions to obtain the best results for your experiments.
Immunogen	A synthesized peptide derived from human MAP2K6.
MW	37kD
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography with SulfoLink™ Coupling Resin was utilized to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure the stability of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to multiple freeze and thaw cycles as it can adversely affect its quality.
Storage buffer	This product contains Rabbit IgG in a solution of phosphate buffered saline pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. It should be stored at a temperature of -20 °C and is expected to remain stable for up to 12 months from the date of receipt.
Target
Background	Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. With MAP3K3/MKK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinases p38 MAPK11, MAPK12, MAPK13 and MAPK14 and plays an important role in the regulation of cellular responses to cytokines and all kinds of stresses. Especially, MAP2K3/MKK3 and MAP2K6/MKK6 are both essential for the activation of MAPK11 and MAPK13 induced by environmental stress, whereas MAP2K6/MKK6 is the major MAPK11 activator in response to TNF. MAP2K6/MKK6 also phosphorylates and activates PAK6. The p38 MAP kinase signal transduction pathway leads to direct activation of transcription factors. Nuclear targets of p38 MAP kinase include the transcription factors ATF2 and ELK1. Within the p38 MAPK signal transduction pathway, MAP3K6/MKK6 mediates phosphorylation of STAT4 through MAPK14 activation, and is therefore required for STAT4 activation and STAT4-regulated gene expression in response to IL-12 stimulation. The pathway is also crucial for IL-6-induced SOCS3 expression and down-regulation of IL-6-mediated gene induction; and for IFNG-dependent gene transcription. Has a role in osteoclast differentiation through NF-kappa-B transactivation by TNFSF11, and in endochondral ossification and since SOX9 is another likely downstream target of the p38 MAPK pathway. MAP2K6/MKK6 mediates apoptotic cell death in thymocytes. Acts also as a regulator for melanocytes dendricity, through the modulation of Rho family GTPases.
Tissue specificity	Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
Posttranslational modification	Weakly autophosphorylated. Phosphorylated at Ser-207 and Thr-211 by the majority of M3Ks, such as MAP3K5/ASK1, MAP3K1/MEKK1, MAP3K2/MEKK2, MAP3K3/MEKK3, MAP3K4/MEKK4, MAP3K7/TAK1, MAP3K11/MLK3 and MAP3K17/TAOK2.Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Celluar localization	Cytoskeleton;Cytosol;Nucleus;
UniPort	P52564