Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-TBK1 Polyclonal Antibody#abs136546

Please note that the price provided is for reference only. For detailed pricing information, please contact our seller Vecent. It's important to reach out to our seller for accurate pricing details. Western blot analysis of extracts of various celllines, using TBK1 ntibody. This product is for...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs136546-100ug	1-2 Weeks	301.0
abs136546-50ug	1-2 Weeks	201.0

Please note that the price provided is for reference only. For detailed pricing information, please contact our seller Vecent. It's important to reach out to our seller for accurate pricing details.

Overview
catalog	abs136546
Description	I-kappa-B (IKB) proteins play a crucial role in inhibiting the NF-kappa-B (NFKB) complex of proteins. These IKB proteins function by trapping NFKB in the cytoplasm, preventing its activation. One of the mechanisms by which IKB proteins exert their inhibitory function is by undergoing phosphorylation on serine residues. This phosphorylation is mediated by IKB kinases, which specifically mark the IKB proteins for degradation through the ubiquitination pathway. By undergoing degradation, the IKB proteins release their grip on NFKB, allowing it to become activated and translocate to the nucleus. Notably, a gene encodes a protein that shares similarities with the IKB kinases. This protein is capable of facilitating NFKB activation in response to specific growth factors.
Other names	TANK-binding kinase 1, also known as NF-kappa-B-activating kinase, NF kB activating kinase, or serine/threonine protein kinase TBK 1, is an enzyme encoded by the FLJ11330 gene in humans. Its other aliases include FTDALS4, NAK, T2K, and TBK1. TBK1 plays a crucial role in the regulation of innate immunity by activating the transcription factor NF-kappa-B. It belongs to the family of serine/threonine protein kinases and mediates the phosphorylation of multiple target proteins involved in inflammatory responses. Understanding the function of TBK1 has important implications for the development of therapies for chronic inflammation and autoimmune disorders.
Source	Rabbit
Specificity	The TBK1 antibody has the ability to recognize and bind to the total TBK1 protein present in an organism at natural levels. This ensures the accurate identification and detection of TBK1 in various biological samples and systems.
Species Reactivity	Human;Mouse;Rat
Antigen	TBK1
Application	The recommended dilutions for using the antibody in different assays are as follows: 1:500 to 1:2000 for Western blotting, 1:50 to 1:200 for immunohistochemistry, 1:100 to 1:500 for immunofluorescence or immunocytochemistry, and finally 1:20000 to 1:40000 for ELISA with peptide. Please note that the actual optimal dilution may vary depending on the sample type, matrix effects, and experimental conditions. It is always important to run appropriate controls and compare the results with suitable positive and negative controls. Proper validation and optimization can increase the sensitivity, specificity, and reproducibility of the antibody-based assays and enhance the scientific validity of the data obtained.
Immunogen	A synthesized peptide derived from human TBK1.
MW	84kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum was subjected to peptide affinity chromatography for purification. A purification process was carried out to extract a highly refined antiserum for use. The method employed peptide affinity chromatography and SulfoLink™ Coupling Resin to successfully achieve the desired outcome. The antiserum was subjected to careful purification to ensure that it was of the highest quality possible. The peptides were isolated and captured using the resin, resulting in a purified antiserum that was ideal for use in various applications.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy (PubMed:21617041). Phosphorylates SMCR8 component of the C9orf72-SMCR8 complex, promoting autophagosome maturation (PubMed:27103069). Phosphorylates and activates AKT1 (PubMed:21464307). Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Attenuates retroviral budding by phosphorylating the endosomal sorting complex required for transport-I (ESCRT-I) subunit VPS37C (PubMed:21270402). Phosphorylates Borna disease virus (BDV) P protein (PubMed:16155125).
Tissue specificity	Ubiquitous with higher expression in testis. Expressed in the ganglion cells, nerve fiber layer and microvasculature of the retina.
Posttranslational modification	Autophosphorylation at Ser-172 activates the kinase, and is an essential step for virus-triggered signaling. Phosphorylated by IKBKB/IKKB at Ser-172. Phosphorylation requires homodimerization and ubiquitination at Lys-30 and Lys-401. Dephosphorylated at Ser-172 by PPM1B and this negatively regulates its role in mediating antiviral response.'Lys-63'-linked polyubiquitination by MIB1 after RNA virus infection, or by NRDP1 after LPS stimulation at Lys-30 and Lys-401, participates in kinase activation. 'Lys-48'-linked polyubiquitination at Lys-670 by DTX4 leads to proteasomal degradation. 'Lys-48'-linked polyubiquitination by TRAIP also leads to proteasomal degradation.
Celluar localization	Cytosol;Endosome;
UniPort	Q9UHD2