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Rabbit Anti-HPRT1 Polyclonal Antibody#abs136975

Please note that the price mentioned above is merely for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Thank you. Western blot analysis of extracts of various celllines, using HPRT1 antibody. This product is for research use...

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Description

Catalog-specification	Delivery time	USD price
abs136975-100ug	1-2 Weeks	301.0
abs136975-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is merely for your reference. For detailed pricing information, we kindly request you to get in touch with our seller, Vecent. Thank you.

Overview
catalog	abs136975
Description	The gene under discussion encodes for a transferase that aids in the conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate by transferring the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme is crucial in the production of purine nucleotides through the purine salvage pathway. Lesch-Nyhan syndrome and gout are the results of mutations in this particular gene.
Other names	HPRT 1, also known as hypoxanthine-guanine phosphoribosyltransferase (HGPRT or HGPRTase), is an enzyme encoded by the HPRT1 gene in humans. This enzyme is involved in the salvage pathway of purine biosynthesis. It catalyzes the conversion of hypoxanthine and guanine to their corresponding nucleotides utilizing phosphoribosyl pyrophosphate (PRPP) as a substrate. HPRT 1 plays a crucial role in the production of guanosine and inosine monophosphate, which are essential for DNA and RNA synthesis. Mutations in the HPRT1 gene can lead to Lesch-Nyhan syndrome, a rare inherited disorder that affects the function of HPRT 1. Individuals with Lesch-Nyhan syndrome exhibit a range of symptoms including neurological abnormalities, self-injurious behaviors, and a tendency towards gout due to the accumulation of uric acid. Understanding the structure and function of HPRT 1 is important for studying purine metabolism and developing therapeutic strategies for Lesch-Nyhan syndrome. Research on this enzyme has provided valuable insights into the intricate biochemical pathways involved in the generation and utilization of purines in the human body.
Source	Rabbit
Specificity	The HPRT1 Antibody is capable of detecting the overall levels of endogenous HPRT1 in a given sample. This indicates that the antibody can identify the total amount of HPRT1 present in the sample that is being analyzed.
Species Reactivity	Human;Mouse;Rat
Antigen	HPRT1
Application	The dilution ranges for WB, IHC, and ELISA (peptide) assays are as follows: WB can be performed using a dilution range of 1:500 to 1:2000. For IHC, the recommended dilutions range from 1:50 to 1:200. ELISA (peptide) assays typically require dilutions in the range of 1:20000 to 1:40000. It is important to note that these dilution ranges are crucial in achieving accurate and reliable results for each assay.
Immunogen	A synthesized peptide derived from human HPRT1.
MW	24kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway.
Celluar localization	Cytosol;Extracellular region or secreted;
UniPort	P00492