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Rabbit Anti-HMGA1 Polyclonal Antibody#abs131313

Please be informed that the price mentioned is only for your reference. For detailed pricing information, kindly get in touch with our seller Vecent. Feel free to reach out for further assistance. Western blot analysis of HMGA1 expression in mouse brain tissue This product is for research use...

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Description

Catalog-specification	Delivery time	USD price
abs131313-100ug	1-2 Weeks	301.0
abs131313-50ug	1-2 Weeks	201.0

Please be informed that the price mentioned is only for your reference. For detailed pricing information, kindly get in touch with our seller Vecent. Feel free to reach out for further assistance.

Overview
catalog	abs131313
Description	The protein group known as HMG-I/Y is known for their strong affinity for the minor groove of double-stranded DNA in A+T rich zones. They are believed to play essential roles in nucleosome phasing and the 3'-end processing of mRNA molecules. Additionally, HMG-I/Y proteins may contribute to gene regulation for those that have or are located in close proximity to A+T-rich regions.
Other names	There are several different isoforms of the high mobility group (nonhistone chromosomal) protein, including I and Y. Another important protein in this family is the high mobility group AT hook 1. Also known as high mobility group AT-hook protein 1, or HMGA1, this protein plays a crucial role in the structure and function of chromatin, the material that makes up chromosomes. Other names for HMGA1 include high mobility group protein 1 and high mobility group protein A1. HMGA1 is also sometimes referred to as high mobility group protein HMG-I/HMG-Y, as it shares similarities with other nonhistone chromosomal high mobility group proteins. This protein has several functions, including the regulation of gene expression and DNA replication. It is also involved in a variety of cellular processes, such as cell differentiation and development. Other members of the high mobility group family include high mobility group protein Ia, high mobility group protein R (also known as HMG R), HMG-I(Y), HMGA1A, and HMGIY. These proteins have similar roles in regulating gene expression and chromatin structure, and are involved in a range of cellular processes. The high mobility group family of proteins is an important area of study for researchers interested in understanding the complex ways in which cells function and develop.
Source	Rabbit
Specificity	The HMGA1 Antibody is capable of detecting the total amount of endogenous HMGA1. This indicates that the antibody can identify all forms of HMGA1 that are naturally occurring in the sample.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Bovine;Dog;Horse;
Antigen	HMGA1
Application	WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human HMGA1.
MW	11 kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification process for the antiserum involved peptide affinity chromatography. This allowed for an efficient and specific separation of the desired antibodies.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
Posttranslational modification	Constitutively phosphorylated on two or three sites. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoforms HMG-I and HMG-Y can be phosphorylated by HIPK2. Phosphorylation of HMG-I at Ser-36, Thr-53 and Thr-78 and of HMG-Y at Thr-42 and Thr-67 by HIPK2 modulates DNA-binding affinity.HMG-Y is not methylated.Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.
Celluar localization	Cytosol;Nucleus;
UniPort	P17096