PICK1 Polyclonal Antibody #abs115593

abs115593-50ul

In Stock

228

Other names

PICK;PRKCABP

Source

Rabbit

Species Reactivity

Human

Application

WB 1:500 - 1:2000

Immunogen

A synthetic peptide of human PICK1

Properties

HL-60,Jurkat,K-562,SH-SY5Y,HT-29,SW620

Properties

Purification

Affinity purification

Clonality

Polyclonal Antibody

Isotype

IgG

Stability & Storage

Store at -20℃. Avoid freeze / thaw cycles.

Storage buffer

Store at -20℃. Avoid freeze / thaw cycles.
A buffer solution is made up of PBS with 0.02% sodium azide, 50% glycerol, and pH7.3. This solution is commonly used in various laboratory processes. To prepare the buffer, you simply mix the PBS solution with the sodium azide, add in the glycerol, and adjust the pH to 7.3. The buffer is then ready to be used in experiments. The sodium azide serves as a preservative to prevent bacterial growth, while the glycerol helps to stabilize the solution. Overall, the buffer ensures that the experiments are performed under controlled conditions, leading to accurate and reliable results.

Target

Background

The gene in question encodes a protein that possesses a PDZ domain, facilitating its interaction with protein kinase C, alpha (PRKCA). Acting as an adaptor, this protein binds to and organizes the subcellular positioning of various membrane proteins. Its complex interactions have been observed with several glutamate receptor subtypes, monoamine plasma membrane transporters, and non-voltage gated sodium channels. Through these interactions, it is believed to regulate the distribution and functionality of PRKCA-bound membrane proteins. Additionally, this protein serves as an anchoring molecule, specifically targeting PRKCA to mitochondria in response to ligand-specific signals. Interestingly, three different transcript variants of this gene have been identified, all of which encode the same protein.

Celluar localization

A synapse is a specialized junction between two cells, specifically between the presynaptic cell membrane and the postsynaptic cell membrane. This connection is crucial for communication in the nervous system. Within the synapse, various structures and molecules play important roles.
The presynaptic cell membrane and the postsynaptic cell membrane are separated by a small gap called the synaptic cleft. The presynaptic cell releases chemical messengers, called neurotransmitters, into the synaptic cleft. These neurotransmitters then bind to receptors on the postsynaptic cell membrane, initiating a response in the postsynaptic cell.
The postsynaptic cell membrane is enriched with protein clusters known as postsynaptic densities. These densities contain numerous proteins involved in signal transduction and receptor clustering. They help to amplify and transmit the synaptic signal into the postsynaptic cell.
The synapse is supported by various cellular components, including the cytoplasm, which fills the cell and provides a supportive environment for cellular processes. The cytoplasm contains organelles and a network of protein fibers called the cytoskeleton, which helps maintain cell shape and facilitates intracellular transport.
Cell junctions, such as tight junctions and gap junctions, are vital in maintaining the structural and functional integrity of cells. They mediate direct communication and coordination between neighboring cells. In the context of a synapse, cell junctions help to anchor the presynaptic and postsynaptic membranes in close proximity, facilitating efficient neurotransmitter release and reception.
Lipid anchors are molecules that attach proteins to the cell membrane. They serve as important mechanisms for localizing peripheral membrane proteins, such as those found in the postsynaptic density, to specific regions of the cell membrane. This spatial organization is crucial for the proper functioning of the synapse.
The perinuclear region, located around the nucleus, is involved in various cellular processes, including the organization and transport of materials within the cell. In the context of the synapse, the perinuclear region may contribute to the synthesis and trafficking of proteins and other molecules essential for synaptic function.
Synaptosomes are small fragments of nerve terminals isolated from the nervous system. They contain all the components necessary for synaptic transmission, including the presynaptic membrane, neurotransmitter vesicles, and postsynaptic receptors. Synaptosomes are commonly used in research to study synaptic function and investigate the molecular mechanisms underlying neurotransmission.
In summary, the synapse is a complex structure involving various components and molecules. It relies on cell junctions, lipid anchors, the cytoplasm, the cytoskeleton, the perinuclear region, and the postsynaptic density to facilitate efficient communication between neurons. Understanding the intricacies of the synapse is crucial for deciphering how information is processed and transmitted in the nervous system.

UniPort

Q9NRD5