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Rabbit Anti-Histone H4K20me3 Polyclonal Antibody#abs136470

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Description

Catalog-specification	Delivery time	USD price
abs136470-100ug	1-2 Weeks	301.0
abs136470-50ug	1-2 Weeks	201.0

Overview
catalog	abs136470
Description	Eukaryotes rely on histones, which are fundamental nuclear proteins, to maintain the nucleosome structure within the chromosomal fiber. These proteins organize approximately 146 base pairs of DNA into a compact structure by wrapping it around an octamer composed of two sets of four core histones (H2A, H2B, H3, and H4). To further condense the chromatin fiber, a linker histone called H1 interacts with the DNA between nucleosomes, forming higher order chromatin structures. Interestingly, this specific gene belonging to the histone H4 family lacks introns and produces transcripts without polyA tails. Instead, these transcripts feature a palindromic termination element. Positioned on chromosome 1, this gene is a member of a histone cluster containing four duplicated histone genes. Notably, the information provided represents the centromeric copy of this gene.
Other names	H4; H4.k; H4/a; H4/b; H4/c; H4/d; H4/e; H4/g; H4/h; H4/I; H4/j; H4/k; H4/m; H4/n; H4/p; dJ160A22.1; dJ160A22.2; dJ221C16.1; dJ221C16.9; FO108; H4FA; H4FB; H4FC; H4FD; H4FE; H4FG; H4FH; H4FI; H4FJ; H4FK; H4FM; H4FN; H4M; HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4; HIST2H4A; Hist4h4; Histone 1 H4a; Histone 1 H4b; Histone 1 H4c; Histone 1 H4d; Histone 1 H4e; Histone 1 H4f; Histone 1 H4h; Histone 1 H4i; Histone 1 H4j; Histone 1 H4k; Histone 1 H4l; Histone 2 H4a; histone 4 H4; Histone H4; MGC24116; I would like to present the following list of proteins and histones: H4, H4.k, H4/a, H4/b, H4/c, H4/d, H4/e, H4/g, H4/h, H4/I, H4/j, H4/k, H4/m, H4/n, H4/p, dJ160A22.1, dJ160A22.2, dJ221C16.1, dJ221C16.9, FO108, H4FA, H4FB, H4FC, H4FD, H4FE, H4FG, H4FH, H4FI, H4FJ, H4FK, H4FM, H4FN, H4M, HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, HIST1H4E, HIST1H4F, HIST1H4H, HIST1H4I, HIST1H4J, HIST1H4K, HIST1H4L, HIST2H4, HIST2H4A, Hist4h4, Histone 1 H4a, Histone 1 H4b, Histone 1 H4c, Histone 1 H4d, Histone 1 H4e, Histone 1 H4f, Histone 1 H4h, Histone 1 H4i, Histone 1 H4j, Histone 1 H4k, Histone 1 H4l, Histone 2 H4a, histone 4 H4, Histone H4, MGC24116. Please note that these proteins and histones play important roles in various biological processes. Their intricate structure and function contribute to the regulation of gene expression and the maintenance of chromatin structure. The study of these molecules provides insights into fundamental biological mechanisms and their dysregulation has been implicated in various diseases. Further research is required to elucidate the specific roles of each protein and histone, paving the way for potential therapeutic interventions.
Source	Rabbit
Specificity	The endogenous levels of total Histone H4K20me3 can be accurately detected using the Histone H4K20me3 Antibody. This antibody is designed to specifically target and bind to Histone H4K20me3, allowing researchers to study and analyze the presence and distribution of this histone modification. By detecting the levels of Histone H4K20me3 in various cellular contexts, this antibody provides valuable insights into epigenetic regulation and its underlying mechanisms. It plays a crucial role in understanding the role of Histone H4K20me3 in chromatin remodeling, gene expression, and other cellular processes. The accurate detection of total Histone H4K20me3 levels using this antibody contributes significantly to the advancement of epigenetic research.
Species Reactivity	Human;Mouse
Antigen	Histone H4K20me3
Application	WB 1:500-1:1000, ELISA(peptide) 1:20000-1:40000
Immunogen	One possible rephrased version could be: A peptide that has been artificially modified to contain methyl groups is designed to mimic the sequence of amino acids located near lysine 20 of human histone H4.
MW	11kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Posttranslational modification	Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by KMT5B and KMT5C and induces gene silencing.Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4.Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).Sumoylated, which is associated with transcriptional repression.Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
Celluar localization	Extracellular region or secreted;Nucleus;
UniPort	P62805