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Rabbit Anti-Histone H3K79me1 Polyclonal Antibody#abs136465

Please bear in mind that the price mentioned here is only for your reference. For the detailed pricing, we request you to kindly get in touch with our seller, Vecent. Western blot analysis of extracts of HeLa cellline and H3 protein expressed in E.coli., usingH3K79me1 antibody|Dot-blot analysis...

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Description

Catalog-specification	Delivery time	USD price
abs136465-100ug	1-2 Weeks	301.0
abs136465-50ug	1-2 Weeks	201.0

Please bear in mind that the price mentioned here is only for your reference. For the detailed pricing, we request you to kindly get in touch with our seller, Vecent.

Overview
catalog	abs136465
Description	The regulation of transcription in eukaryotes relies heavily on the modulation of chromatin structure. Chromatin, composed of DNA wrapped around eight core histone proteins (two each of H2A, H2B, H3, and H4), forms the fundamental unit known as the nucleosome (1). Post-translational modifications of the N-terminal tails of core histones, such as acetylation, phosphorylation, methylation, and ubiquitination, play a crucial role in this process (2-5). These modifications are induced by various stimuli and directly impact the accessibility of chromatin to transcription factors, thereby influencing gene expression (6). Among these modifications, histone H2B is predominantly acetylated at Lys5, 12, 15, and 20 in most species (4, 7). Histone H3, on the other hand, is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Notably, acetylation of H3 at Lys9 seems to play a dominant role in histone deposition and chromatin assembly in certain organisms (2, 3). During both mitosis and meiosis, phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is closely associated with chromosome condensation (8-10). Additionally, phosphorylation of Thr3 of histone H3, catalyzed by the kinase haspin, is highly conserved across many species. Immunostaining with phospho-specific antibodies in mammalian cells has revealed that Thr3 of H3 undergoes mitotic phosphorylation in prophase and subsequent dephosphorylation during anaphase (11).
Other names	The HIST1 cluster is a prominent family of genes that encode for the histone H3 protein. There are various members of this family, including H3E, H3.1, H3FF, and H3FL, among others. These genes are responsible for the production of H3 histones, which are important components of chromatin and play a significant role in gene expression and epigenetic regulation. In humans, there are several different types of histone H3 proteins, including H3A, H3B, H3C, H3D, H3E, H3F, H3G, H3H, H3I, and H3J. Each of these proteins has a unique function and is expressed in different tissues throughout the body. Interestingly, the HIST1 cluster has been linked to a variety of human diseases, including cancer, developmental disorders, and neurological disorders. This highlights the importance of studying these genes and understanding their role in human health and disease. Overall, the histone H3 family is a crucial component of gene expression and epigenetic regulation. The HIST1 cluster, in particular, plays a critical role in encoding for these proteins and has been linked to various human diseases. Further research into these genes and their function is essential for understanding human health and disease.
Source	Rabbit
Specificity	Histone H3K79me1 Antibody detects endogenous levels of total Histone H3K79me1.
Species Reactivity	Human;Mouse
Antigen	Histone H3K79me1
Application	WB 1:500-1:2000, IHC 1:50-1:200, IF 1:50-1:200, IP 1:50-1:200, CHIP 1:50-1:200, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthetic methylated peptide corresponding to residues surrounding K79 of human histone H3.
MW	15kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLinkâ„¢ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 Â°C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 Â°C.Stable for 12 months from date of receipt.
Target
Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Tissue specificity	Expressed in testicular cells.
Posttranslational modification	Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability (By similarity).Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters (By similarity).Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication (By similarity).Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin (By similarity).Ubiquitinated.Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression (By similarity).
Celluar localization	Extracellular region or secreted;Nucleus;
UniPort	Q16695

Western blot analysis of extracts of HeLa cellline and H3 protein expressed in E.coli., usingH3K79me1 antibody|Dot-blot analysis of all sorts of methylation peptides using H3K79me1 antibody|Immunofluorescence analysis of 293T cell using H3K79me1 antibody. Blue: DAPI for nuclear staining.

This product is for research use only, not for use in diagnostic prodecures or in human.

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