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Rabbit Anti-Histone H3K36me3 Polyclonal Antibody#abs136464

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Description

Catalog-specification	Delivery time	USD price
abs136464-100ug	1-2 Weeks	301.0
abs136464-50ug	1-2 Weeks	201.0

Overview
catalog	abs136464
Description	The regulation of transcription in eukaryotes heavily relies on the modulation of chromatin structure. The fundamental unit of chromatin is the nucleosome, which comprises eight core histone proteins (two each of H2A, H2B, H3, and H4) coiled around DNA (1). Post-translational modifications such as acetylation, phosphorylation, methylation, and ubiquitination of the amino-terminal tails of core histones play a critical role in modulating gene expression by altering chromatin accessibility to transcription factors (2-5). Typically, H2B is acetylated at specific lysine residues 5, 12, 15, and 20, while H3 is acetylated at lysine 9, 14, 18, 23, 27, and 56. Notably, acetylation at lysine 9 of H3 appears to play a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Meanwhile, phosphorylation of H3 at serine 10, 28, and threonine 11 correlates tightly with chromosomal condensation during mitosis and meiosis (8-10), whereas threonine 3 of H3 is conserved among many species and is catalyzed by haspin kinase (11). Phospho-specific antibodies staining of mammalian cells reveals mitotic phosphorylation of H3 threonine 3 during prophase, followed by its dephosphorylation in anaphase (11).
Other names	The H3 gene family cluster contains several members, including H3A, H3B, H3C, H3D, H3E, H3F, H3G, H3H, H3I, and H3J. These genes all code for histone proteins, which are involved in packaging DNA into chromosomes. There are also different variants of H3 proteins, such as H3.1, H3.2, and H3.3, which have slightly different functions and play roles in various biological processes. Additionally, there are different aliases for these genes, such as H3E, H3FF, and H3FL. Studies have shown that mutations in some H3 genes can lead to a variety of diseases, including cancer and developmental disorders. While the specific functions of each H3 gene are still being studied, it is clear that these genes play important roles in regulating gene expression and maintaining genomic stability.
Source	Rabbit
Specificity	Histone H3K36me3 Antibody detects endogenous levels of total Histone H3K36me3.
Species Reactivity	Human;Mouse;Rat
Antigen	Histone H3K36me3
Application	WB 1:500–1:2000, IF 1:50–1:200, CHIP 1:50–1:200, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthetic tri-methylated peptide corresponding to residues surrounding K36of human histone H3.
MW	15kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Tissue specificity	Expressed in testicular cells.
Posttranslational modification	Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability (By similarity).Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters (By similarity).Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication (By similarity).Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin (By similarity).Ubiquitinated.Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression (By similarity).
Celluar localization	Extracellular region or secreted;Nucleus;
UniPort	Q16695