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Rabbit Anti-HIC-2 Polyclonal Antibody#abs138076

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Description

Catalog-specification	Delivery time	USD price
abs130076-100ug	1-2 Weeks	301.0
abs130076-50ug	1-2 Weeks	201.0

Overview
catalog	abs130076
Description	CBP is a protein acetyltransferase that is capable of activating histones transcriptionally. It has been observed to acetylate the NCOA3 coactivator and bind specifically to phosphorylated CREB1, thereby enhancing its transcriptional activity towards cAMP-responsive genes. CARM1 methylation of the KIX domain has been found to block association with CREB, leading to a blockade of CREB signaling and subsequent activation of an apoptotic response. Studies have found that CBP is present in a complex with various proteins, including NCOA2, NCOA3, IKKA, IKKB, and IKBKG. It is also believed to be part of a complex with HIF1A and EP300.
Other names	CREB binding protein, also known as CBP, is a human protein that plays a crucial role in gene regulation. It binds to the CREB protein, which is involved in the cellular response to cAMP signals. CBP acts as a coactivator and enhances the transcriptional activity of CREB, promoting the expression of genes involved in various cellular processes. One of the notable functions of CBP is its involvement in the Rubinstein-Taybi syndrome (RSTS), a rare genetic disorder characterized by physical and intellectual abnormalities. Mutations in the CBP gene have been identified as one of the causes of RSTS. These mutations lead to a deficiency or dysfunction of CBP, affecting the normal gene expression patterns and causing the characteristic features of the syndrome. CBP, also referred to as KAT3A, exhibits histone acetyltransferase activity, modifying the structure of chromatin and promoting gene activation. This protein interacts with a variety of transcription factors and coactivators to regulate gene expression. Its multiple functional domains enable it to participate in various protein-protein interactions, allowing for the assembly of large protein complexes involved in transcriptional regulation. Understanding the role of CBP is critical in elucidating the molecular mechanisms underlying gene expression regulation. Further research on CBP and its interactions with other molecules will not only expand our knowledge of fundamental biological processes but also contribute to unraveling the complex pathologies associated with genetic disorders like RSTS.
Source	Rabbit
Specificity	The antibody for CREB-BP can identify the complete amount of endogenous CREB-BP.
Species Reactivity	Human;Mouse
Antigen	CREB-BP
Application	Western blotting is typically performed at dilutions ranging from 1:500 to 1:3000, while immunohistochemistry is usually conducted at dilutions of 1:50 to 1:200. For immunofluorescence/immunocytochemistry, dilutions of 1:100 to 1:500 are commonly used. In the case of ELISA using peptides as antigens, high dilutions ranging from 1:20000 to 1:40000 are typically employed. It is important to ensure appropriate dilution ranges for each technique to achieve accurate and reliable results.
Immunogen	A synthesized peptide derived from human CREB-BP.
MW	265kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was performed through peptide affinity chromatography. Please rearrange the content to generate a highly similar version while ensuring that the information remains based on the original text.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK-ARNTL/BMAL1 heterodimers. Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902).
Posttranslational modification	Methylation of the KIX domain by CARM1 blocks association with CREB. This results in the blockade of CREB signaling, and in activation of apoptotic response (By similarity).Phosphorylated by CHUK/IKKA at Ser-1382 and Ser-1386; these phosphorylations promote cell growth by switching the binding preference of CREBBP from TP53 to NF-kappa-B.Sumoylation negatively regulates transcriptional activity via the recruitment of DAAX.Autoacetylation is required for binding to protein substrates, such as acetylated histones and acetylated TP53/p53.
Celluar localization	Nucleus;
UniPort	Q92793