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Rabbit Anti-PAK5/6 Polyclonal Antibody#abs140503

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Description

Catalog-specification	Delivery time	USD price
abs140503-100ug	1-2 Weeks	301.0
abs140503-50ug	1-2 Weeks	201.0

Overview
catalog	abs140503
Other names	p21-activated kinase 7, also known as PAK7, is a protein kinase encoded by the KIAA1264 gene. It is classified under the EC number 2.7.11.1 and is also referred to as MGC26232. PAK7 belongs to the family of p21-activated kinases and is closely related to PAK5. PAK7 plays a crucial role in various cellular processes, particularly in cell proliferation, migration, and survival. It is activated by binding to the p21 protein (Cdc42/Rac), which further leads to the activation of downstream signaling cascades. PAK7 is involved in the regulation of gene expression and cytoskeletal rearrangements. Another member of the p21-activated kinase family is PAK6, which is encoded by the CDKN1A gene. PAK6 shares similarities with PAK7 in terms of function and structure. It is classified under the EC number 2.7.1.37 and is also known as p21-activated protein kinase 6. PAK6 plays a role in signaling pathways involved in cell growth and differentiation. It is activated by the p21 protein (Cdc42/Rac) and contributes to the regulation of gene expression and cellular processes. Both PAK5 and PAK6 are serine/threonine protein kinases and are crucial in mediating cellular responses to extracellular signals. They are potential targets for therapeutic interventions, particularly in diseases where abnormal cell proliferation and migration are observed. In summary, PAK7 and PAK6 are closely related members of the p21-activated kinase family. They play essential roles in cell signaling pathways and are involved in regulating diverse cellular processes. Understanding their functions and mechanisms of activation can provide insights into potential therapeutic strategies for various diseases.
Source	Rabbit
Specificity	The detection of endogenous levels of total PAK5/6 is possible with the PAK5/6 Antibody. This antibody has the ability to identify and measure the presence of PAK5/6 proteins in the cell. By using this antibody, researchers can gain insights into the expression and regulation of these proteins, contributing to a better understanding of cellular signaling pathways.
Species Reactivity	Human
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Bovine;Horse;
Antigen	PAK5/6
Application	One possible variation of the original content is: For Western blotting, it is recommended to use a dilution factor of 1:1000. For immunofluorescence/immunocytochemistry, a dilution range of 1:100-1:500 is suitable. When performing ELISA with the peptide of interest, a dilution factor of 1:20000-1:40000 is advised. These dilution ranges have been optimized to achieve optimal signal-to-noise ratio and minimize background signals. It is important to carefully titrate the primary antibody to find the optimal dilution that yields specific and strong staining.
Immunogen	A synthesized peptide derived from human PAK5/6.
MW	75kDa
Properties
Concentration	1mg/ml
purification	Through the process of peptide affinity chromatography using SulfoLink™ Coupling Resin, the antiserum was efficiently purified.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions.
Tissue specificity	Predominantly expressed in brain.
Posttranslational modification	Autophosphorylated when activated by CDC42/p21.
Celluar localization	Mitochondrion;Nucleus;
UniPort	Q9P286/Q9NQU5