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Rabbit Anti-GOLGA2 Polyclonal Antibody#abs136798

Please note that the price provided is for your reference only. For detailed pricing information, kindly get in touch with our seller Vecent. We value your understanding. Western blot analysis of extracts of various celllines, using RAD51 antibody. This product is for research use only, not for...

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Description

Catalog-specification	Delivery time	USD price
abs136798-100ug	1-2 Weeks	301.0
abs136798-50ug	1-2 Weeks	201.0

Please note that the price provided is for your reference only. For detailed pricing information, kindly get in touch with our seller Vecent. We value your understanding.

Overview
catalog	abs136798
Description	The Golgi apparatus is a crucial organelle involved in the modification and transportation of proteins and lipids within the secretory pathway. It is composed of a series of flattened membrane sacs called cisternae, which are stacked together. During mitosis, the Golgi fragments, and its reorganization is believed to rely on its interactions with microtubules. Within the Golgi, there are proteins called golgins, and this specific gene encodes one of these golgins. The primary role of this protein is thought to be involved in the organization of Golgi cisternae and the transportation of vesicles. Variants of this gene have been identified through alternative splicing, but their complete structure remains unknown.
Other names	The GM130 protein, also known as Golgi matrix protein GM130 or golgin A2, is a cis Golgi matrix protein with a molecular weight of 130 kDa. It is an autoantigen and a member of the golgin subfamily A. GM130, also referred to as Golgin 95 or Golgi autoantigen, plays a crucial role in maintaining the structure and function of the Golgi apparatus. Its presence and localization within the cis-Golgi compartment contribute to intra-Golgi transport and protein trafficking. The gene encoding GM130 is called Golga2 or Golgin-95, and it is also known as SY11 protein or MGC20672. Overall, GM130 is a key protein involved in Golgi organization and membrane trafficking.
Source	Rabbit
Specificity	The GOLGA2 Antibody is capable of detecting total GOLGA2 levels that occur naturally within the body. To create new content that accurately reflects this information, one could rearrange the original statement while maintaining its meaning. So, a similar statement might read: "Endogenous levels of total GOLGA2 can be detected using the GOLGA2 Antibody, indicating its efficacy in identifying naturally occurring GOLGA2."
Species Reactivity	Human;Mouse;Rat
Antigen	GOLGA2
Application	The dilutions for Western blot range from 1:500 to 1:2000, for immunohistochemistry from 1:50 to 1:200, and for ELISA (peptide) from 1:20000 to 1:40000. It is important to note that this content has been generated using a language model and may differ significantly from the original text.
Immunogen	A synthesized peptide derived from human GOLGA2.
MW	130kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane. Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly: phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed:26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle: upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed:19242490, PubMed:26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed:18045989, PubMed:19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed:16489344, PubMed:17314401).
Posttranslational modification	Cleaved by caspases at the onset of apoptosis.Methylation by PRMT5 is required for Golgi ribbon formation. While dimethylation at Arg-30 and Arg-35 are confirmed in vivo, it is unclear whether Arg-18 is methylated in vivo.Phosphorylated at Ser-37 by CDK1 at the onset of mitosis, inhibiting the interaction with p115/USO1 and triggering Golgi disassembly (PubMed:20421892, PubMed:26165940). Phosphorylated at Ser-37 in prophase as the Golgi complex starts to break down, and remains phosphorylated during further breakdown and partitioning of the Golgi fragments in metaphase and anaphase. In telophase, GM130 is dephosphorylated by PP2A as the Golgi fragments start to reassemble (By similarity).
Celluar localization	Cytoskeleton;Golgi apparatus;Nucleus;
UniPort	Q08379