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Rabbit Anti-CFLAR Polyclonal Antibody#abs136530

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Description

Catalog-specification	Delivery time	USD price
abs136530-100ug	1-2 Weeks	301.0
abs136530-50ug	1-2 Weeks	201.0

Overview
catalog	abs136530
Description	Cellular FLIP (FLICE inhibitory protein) is a crucial regulator of apoptosis, known by several names including c-FLIP, Casper, CLARP, FLAME, I-FLICE, MRIT, CASH, and Usurpin. It exists in two splice isoforms: FLIP short (FLIPS) and FLIP long (FLIPL). FLIPS contains two death effector domains (DEDs) resembling those present in the death receptor adaptor protein FADD and caspase-8's pro-domain. On the other hand, FLIPL shares significant similarity with caspase-8 (FLICE) and encompasses an additional death effector domain, but lacks the catalytic active site found in caspases and lacks protease activity. Both FLIP isoforms have been found to interact with FADD and pro-caspase-8. The role of FLIP in apoptosis is a matter of debate as some studies suggest its anti-apoptotic nature, while others assert it to be pro-apoptotic. Overexpression of FLIPL can result in caspase-8 heterodimers that generate an active protease, ultimately leading to apoptosis. However, at normal physiological levels, binding of FLIP to FADD's DED is believed to inhibit caspase-8 processing. Decreasing FLIP levels through siRNA or gene targeting renders cells more susceptible to apoptosis triggered by death receptors. Additionally, FLIP has been associated with the resistance of cancer cells to apoptosis and is upregulated in certain cancer types, including Hodgkin's lymphoma, ovarian cancer, and colon carcinomas (9).
Other names	One of the most talked-about proteins in the field of cell biology is c-FLIP. Apart from its different variants, such as c-FLIPL, c-FLIPR, and c-FLIPS, it is also known as CFLA or cellular FLICE-like inhibitory protein. This protein plays a significant role in regulating apoptosis or programmed cell death. Another protein that is often mentioned in the same breath as c-FLIP is CASP8 and FADD-like apoptosis regulator or CASP8AP1. It is also known as Caspase homolog, Caspase-related inducer of apoptosis, and Caspase-like apoptosis regulatory protein, among others. CASP8 and FADD-like apoptosis regulator subunit p12 is another variant of this protein. Caspase-like apoptosis regulatory protein is also referred to as FLAME-1 or FLAME1, and FADD-like antiapoptotic molecule 1 is abbreviated as FLIP2 or I-FLICE. Similarly, MRIT or MACH-related inducer of toxicity is an alternative name for c-FLIP. Also, there is a protein known as Caspase-eight-related protein or Caspase-related induced of apoptosis. Its other names include OTTHUMP00000163715, Usurpin beta, and Usurpin. Last but not least, FADD-like antiapoptotic molecule is also called FLAME or inhibitor of FLICE. In conclusion, these proteins may go by many names, but their critical functions in regulating apoptosis remain the same. They are vital pieces of the puzzle in understanding the process of programmed cell death, and further research on them may lead to the development of new therapies for various diseases.
Source	Rabbit
Specificity	CFLAR Antibody detects endogenous levels of total CFLAR.
Species Reactivity	Human;Mouse;Rat
Antigen	CFLAR
Application	WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human CFLAR.
MW	52kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. Acts as an inhibitor of TNFRSF6 mediated apoptosis. A proteolytic fragment (p43) is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. Lacks enzymatic (caspase) activity.
Tissue specificity	Widely expressed. Higher expression in skeletal muscle, pancreas, heart, kidney, placenta, and peripheral blood leukocytes. Also detected in diverse cell lines. Isoform 8 is predominantly expressed in testis and skeletal muscle.
Posttranslational modification	Proteolytically processed; probably by caspase-8. Processing likely occurs at the DISC and generates subunit p43 and p12.
Celluar localization	Cytosol;Plasma Membrane;
UniPort	O15519