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Rabbit Anti-FCGR3A Polyclonal Antibody#abs136527

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Description

Catalog-specification	Delivery time	USD price
abs136527-100ug	1-2 Weeks	301.0
abs136527-50ug	1-2 Weeks	201.0

Overview
catalog	abs136527
Description	This particular gene, known as FCGR3A, plays a crucial role in immune function by encoding a receptor that specifically recognizes the Fc portion of immunoglobulin G (IgG). Its main function involves the elimination of antigen-antibody complexes from the bloodstream and participation in various antibody-dependent responses. Interestingly, FCGR3A shares a high degree of similarity with FCGR3B, a neighboring gene positioned on chromosome 1. Although both genes encode receptors, FCGR3A is predominantly expressed on natural killer (NK) cells as a glycoprotein firmly embedded in the cell membrane through a transmembrane peptide. On the other hand, FCGR3B finds expression on polymorphonuclear neutrophils (PMN) and is anchored to the cell membrane via a phosphatidylinositol (PI) linkage. Mutations occurring in this gene have been closely associated with a susceptibility to recurring viral infections, systemic lupus erythematosus (an autoimmune disease), and alloimmune neonatal neutropenia. These conditions highlight the significance of FCGR3A in regulating the immune response and maintaining optimal health. Furthermore, it is worth noting that alternative splicing of the gene's transcript has given rise to multiple isoforms, each encoding a slightly different version of the receptor protein. This diversification broadens the functional repertoire of FCGR3A, enhancing its ability to interact with various antibodies and fine-tune immune responses accordingly. Overall, the intricate nature of this gene and its receptor underscores its critical role in immunity.
Other names	CD16a, also known as Fc gamma receptor IIIa or FCGRIIIA, is a cell surface protein that plays a crucial role in immune responses. It is a low affinity immunoglobulin gamma Fc region receptor and is primarily expressed on natural killer (NK) cells, macrophages, and neutrophils. The Fc fragment of IgG, low affinity IIIa receptor (CD16a) is involved in the recognition and binding of immunoglobulin G (IgG) antibodies. It serves as a bridge between the immune cells and IgG-coated pathogens or cells. CD16a is crucial for antibody-dependent cell-mediated cytotoxicity (ADCC), which is an important mechanism for eliminating target cells or pathogens. In addition to CD16a, there is another subtype called CD16b, also known as Fc gamma receptor IIIb or FCGRIIIB. CD16b is mainly expressed on neutrophils and is involved in the activation and recruitment of these cells during inflammatory responses. The Fc gamma receptors, including CD16a and CD16b, are part of the Fc receptor family. They are composed of two subunits, Fc gamma RIIIa (CD16a) and Fc gamma RIIIb (CD16b). These receptors have different affinities for IgG antibodies and are important mediators of immune cell functions. Overall, the CD16 receptors play a crucial role in immune surveillance and defense mechanisms. They contribute to the elimination of target cells or pathogens through antibody-mediated mechanisms. Understanding the functions and regulation of CD16a and CD16b is important for developing therapeutic interventions for immune-related diseases and infections.
Source	Rabbit
Specificity	FCGR3A Antibody detects endogenous levels of total FCGR3A.
Species Reactivity	Human;Mouse;Rat
Antigen	FCGR3A
Application	WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human FCGR3A.
MW	29kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Receptor for the Fc region of IgG. Binds complexed or aggregated IgG and also monomeric IgG. Mediates antibody-dependent cellular cytotoxicity (ADCC) and other antibody-dependent responses, such as phagocytosis.
Tissue specificity	Expressed on natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placental trophoblasts.
Posttranslational modification	Glycosylated. Contains high mannose- and complex-type oligosaccharides. Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms.The soluble form is produced by a proteolytic cleavage.
Celluar localization	Extracellular region or secreted;Plasma Membrane;
UniPort	P08637