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Rabbit Anti-GSTT1 Polyclonal Antibody#abs135905

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Description

Catalog-specification	Delivery time	USD price
abs135905-100ug	1-2 Weeks	301.0
abs135905-50ug	1-2 Weeks	201.0

Overview
catalog	abs135905
Description	The GST superfamily comprises several subfamilies, and each subfamily has unique members with different functions. GSTP1, a member of the Pi subfamily, is primarily involved in the conjugation of reduced glutathione to hydrophobic electrophiles. GSTM1, a cytoplasmic protein, belongs to the Mu family and performs similar functions as GSTP1-1. GSTT1, a cytoplasmic homodimer in the Theta family, is expressed in erythrocytes and actively participates in glutathione conjugation. Additionally, GSTT1 exhibits glutathione peroxidase activity when exposed to cumene hydroperoxide. Overall, each member of the GST superfamily has unique biochemical roles in cellular metabolism and plays a crucial function in the cellular detoxification process.
Other names	Glutathione S transferase 5, also known as GST 5 5 or GST CL1, is an enzyme that plays a critical role in the body's detoxification processes. Specifically, this enzyme is part of the glutathione S-transferase family, which catalyzes the conjugation of glutathione to various electrophilic compounds. One isoform of this enzyme, Glutathione S transferase theta 1 (GSTT1), is particularly well-studied in humans. It is known to be involved in the metabolism of drugs and environmental pollutants, and is also thought to play a role in protecting against oxidative stress. Despite its importance, the exact functions of GSTT1 and other members of the glutathione S-transferase family are not yet fully understood. However, ongoing research in this area holds promise for developing new therapies for a wide range of diseases and conditions.
Source	Rabbit
Specificity	The total GSTT1 can be detected by the GSTT1 antibody, which specifically detects the endogenous levels of this protein.
Species Reactivity	Human;Mouse;Rat
Antigen	GSTT1
Application	The recommended dilution ratios for using this antibody are as follows: for Western Blotting, dilute at 1:500 to 1:2000; for Immunohistochemistry, dilute at 1:50 to 1:200; and for ELISA using peptide, dilute at 1:20000 to 1:40000. These dilutions will provide optimal results for detecting the target protein. It is important to follow these guidelines in order to obtain accurate and reliable results.
Immunogen	A synthesized peptide derived from human GSTT1.
MW	27kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. To ensure the highest level of precision, the process was carefully carried out to produce a purified antiserum that is of the utmost quality and efficacy.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide.
Tissue specificity	Found in erythrocyte. Expressed at low levels in liver. In lung, expressed at low levels in Clara cells and ciliated cells at the alveolar/bronchiolar junction. Absent from epithelial cells of larger bronchioles.
Celluar localization	Cytosol;Extracellular region or secreted;
UniPort	P30711