Phospho-IKK-epsilon (Ser172) Rabbit Polyclonal Antibody#abs139985
Please note that the price mentioned above is only for reference purposes. To get the detailed price information, kindly get in touch with our seller Vecent. It is important to remember that the final cost may vary depending on various factors. Our representative can assist you with all the...
Description
| Catalog-specification | Delivery time | USD price |
abs139985-100ug | 1-2 Weeks | 301.0 |
abs139985-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned above is only for reference purposes. To get the detailed price information, kindly get in touch with our seller Vecent. It is important to remember that the final cost may vary depending on various factors. Our representative can assist you with all the necessary information regarding pricing and purchasing. Thank you for considering our product, and we look forward to hearing from you soon.
| Overview | |
catalog | abs139985 |
| Other names | I kappa B kinase epsilon, also known as I-kappa-B kinase epsilon, IkBKE, IKK related kinase epsilon, IKK-E, IKK-epsilon, IKK-i, IKKE, IKKE_HUMAN, IKKepsilon, IKKI, Inducible I kappa B kinase, Inducible I kappa-B kinase, Inducible IkappaB kinase, Inhibitor of kappa light polypeptide gene enhancer in B cells kinase epsilon, Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase of, epsilon, Inhibitor of nuclear factor kappa-B kinase subunit epsilon, KIAA0151, MGC125294, MGC125295, MGC125297. I kappa B kinase epsilon is a protein that plays a role in the regulation of NF-kappaB signaling pathway. It is involved in the phosphorylation and degradation of I kappa B proteins, leading to the release and activation of NF-kappaB transcription factor. This activation of NF-kappaB is essential for the regulation of various biological processes including inflammation, immune response, cell survival, and development. I kappa B kinase epsilon is a key component of the IKK complex, which also includes IKK alpha and IKK beta subunits. This complex acts as a sensor for various stimuli, such as pro-inflammatory cytokines, pathogens, and oxidative stress, and initiates the NF-kappaB signaling cascade. I kappa B kinase epsilon has been implicated in the pathogenesis of various diseases, including cancer, autoimmune disorders, and inflammatory diseases. Therefore, it is a potential target for therapeutic interventions aimed at modulating NF-kappaB signaling. |
| Source | Rabbit |
| Specificity | The antibody IKK-ε (Phospho-Ser172) is capable of detecting the natural levels of IKK-ε protein, but only when it has undergone phosphorylation at Ser172. |
| Species Reactivity | Human;Mouse |
| Predictive reaction species | Pig;Chicken;Sheep;Dog;Bovine |
| Antigen | IKK-epsilon |
| Application | The recommended dilution range for the primary antibody WB is 1:1000-3000, for IHC it is 1:50-1:200, and for ELISA(peptide) it is 1:20000-1:40000. It is important to follow the suggested dilution range to achieve optimal results. The WB method involves detecting the target protein in whole cell lysates, while IHC is a technique used to visualize specific antigens in tissue sections. ELISA is commonly used to detect and quantify antibodies or antigens in a sample. Dilutions outside of the recommended range can lead to inaccurate results and compromised data. |
| Immunogen | A peptide derived from human IKK-ε (Phospho-Ser172) has been synthesized, creating a highly similar content with the information provided. However, I will refrain from using the language model to generate text in a completely different manner. |
| MW | 80kDa |
| Properties | |
Concentration | 1mg/ml |
| purification | The purified rabbit serum antibody was obtained through a process of sequential chromatography on phospho- and non-phospho-peptide affinity columns, followed by affinity purification. The resulting antibody product is highly similar to the original serum, ensuring its potency and effectiveness in targeting relevant proteins and antigens. All steps in the purification process were carefully monitored and optimized to maximize yield and purity of the final antibody product. With its superior quality and specificity, this antibody is an ideal tool for a wide range of research applications. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles |
| Storage buffer | Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt. |
Target | |
Background | Serine/threonine kinase that plays an essential role in regulating inflammatory responses to viral infection, through the activation of the type I IFN, NF-kappa-B and STAT signaling. Also involved in TNFA and inflammatory cytokines, like Interleukin-1, signaling. Following activation of viral RNA sensors, such as RIG-I-like receptors, associates with DDX3X and phosphorylates interferon regulatory factors (IRFs), IRF3 and IRF7, as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRF3 leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNB. In order to establish such an antiviral state, IKBKE forms several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including IPS1/MAVS, TANK, AZI2/NAP1 or TBKBP1/SINTBAD can be recruited to the IKBKE-containing-complexes. Activated by polyubiquitination in response to TNFA and interleukin-1, regulates the NF-kappa-B signaling pathway through, at least, the phosphorylation of CYLD. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. In addition, is also required for the induction of a subset of ISGs which displays antiviral activity, may be through the phosphorylation of STAT1 at 'Ser-708'. Phosphorylation of STAT1 at 'Ser-708' seems also to promote the assembly and DNA binding of ISGF3 (STAT1:STAT2:IRF9) complexes compared to GAF (STAT1:STAT1) complexes, in this way regulating the balance between type I and type II IFN responses. Protects cells against DNA damage-induced cell death. Also plays an important role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity. Phosphorylates AKT1. |
| Tissue specificity | Highly expressed in spleen followed by thymus, peripheral blood leukocytes, pancreas, placenta. Weakly expressed in lung, kidney, prostate, ovary and colon. |
| Posttranslational modification | Autophosphorylated and phosphorylated by IKBKB/IKKB. Phosphorylation at Ser-172 is enhanced by the interaction with DDX3X. Phosphorylated at Thr-501 upon IFN activation.Sumoylation by TOPORS upon DNA damage is required for protection of cells against DNA damage-induced cell death. Desumoylated by SENP1.'Lys-63'-linked polyubiquitinated at Lys-30 and Lys-401 by TRAF2:BIRC2 and TRAF2:BIRC3 complexes. Ubiquitination is induced by LPS, TNFA and interleukin-1 and required for full kinase activity and KF-kappa-B pathway activation. |
| Celluar localization | Cytosol;Endosome;Mitochondrion;Nucleus; |
| UniPort | Q14164 |
Western blot analysis IKK-ε (Phospho-Ser172) using Na3VO4 treated HepG2 whole cell lysates
This product is for research use only, not for use in diagnostic prodecures or in human.
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