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Rabbit Anti-VAMP2 Polyclonal Antibody#abs135901

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Description

Catalog-specification	Delivery time	USD price
abs135901-100ug	1-2 Weeks	301.0
abs135901-50ug	1-2 Weeks	201.0

Overview
catalog	abs135901
Description	Vesicle-associated membrane protein 2 (VAMP2), also known as synaptobrevin, plays a crucial role in the R-soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex (1). This complex is responsible for facilitating vesicular transport and membrane fusion processes, which are tightly regulated by calcium (2). Interestingly, VAMP2 is primarily found in presynaptic vesicle membranes within neurons. The assembly of VAMP2 with other SNARE proteins, such as syntaxin 1 and SNAP25, is a critical step in enabling membrane fusion and the subsequent release of neurotransmitters (2). However, the significance of VAMP2 extends beyond neuronal functions. It is also involved in granule exocytosis in neutrophils (3), as well as the release of bioactive peptides from cardiac myocytes (4) and juxtaglomerular cells (5). These diverse roles highlight the multifaceted nature of VAMP2 and its importance in various cellular processes.
Other names	SYB; SYB2; Vesicle-associated membrane protein 2 (synaptobrevin 2); FLJ11460; RATVAMPB; RATVAMPIR; Synaptobrevin 2; Vesicle associated membrane protein 2; Vamp2; VAMP2_HUMAN; VAMP 2; Vesicle-associated membrane protein 2; VAMP-2; Vesicle-associated membrane protein 2; Vamp2; Vesicle-associated membrane protein 2; SYB2; Synaptobrevin-2; Synaptobrevin 2; RATVAMPB; RATVAMPIR; VAMP2_HUMAN;.
Source	Rabbit
Specificity	The total VAMP2 levels within the organism can be detected using the VAMP2 Antibody, which is capable of identifying the endogenous amounts of this protein. To elaborate, this antibody specifically targets and recognizes the complete VAMP2 protein present in the organism. By utilizing this antibody, researchers can effectively measure and analyze the levels of total VAMP2 in various biological samples. It is important to note that this antibody is designed to accurately detect the endogenous levels of VAMP2 protein, providing valuable insights into its expression and potential functional roles.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Bovine;Rabbit
Antigen	VAMP2
Application	The dilution ratios for Western blotting (WB) range from 1:500 to 1:2000. For immunohistochemistry (IHC), the recommended dilution ratios are 1:50 to 1:200. ELISA using peptide antigens typically requires dilutions of 1:20000 to 1:40000. It is important to follow these dilution guidelines to ensure accurate and reliable results in your experiments.
Immunogen	A synthesized peptide derived from human VAMP2.
MW	18kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Involved in the targeting and/or fusion of transport vesicles to their target membrane. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1.
Tissue specificity	Nervous system and skeletal muscle.
Posttranslational modification	Phosphorylated by PRKCZ in vitro and this phosphorylation is increased in the presence of WDFY2.
Celluar localization	Cytosol;Extracellular region or secreted;Golgi apparatus;Plasma Membrane;
UniPort	P63027