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Rabbit Anti-EIF4EBP1 Polyclonal Antibody#abs135900

Description

Catalog-specification	Delivery time	USD price
abs135900-100ug	1-2 Weeks	301.0
abs135900-50ug	1-2 Weeks	201.0

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Overview
catalog	abs135900
Description	The protein 4E-BP1, also known as PHAS-1, is a repressor of translation. Its main function is to inhibit cap-dependent translation by binding to the translation initiation factor eIF4E. This interaction can be disrupted by hyperphosphorylation of 4E-BP1, leading to the activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase are involved in regulating the activity of 4E-BP1. In vivo, multiple residues of 4E-BP1 can undergo phosphorylation. Despite being phosphorylated by FRAP/mTOR at Thr37 and Thr46, the binding of 4E-BP1 to eIF4E is not affected. However, it is believed that this phosphorylation primes 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70.
Other names	4E-BP1, also known as eIF4E binding protein 1 or eIF4E-binding protein 1, is a human protein encoded by the gene 4EBP1. This protein, also referred to as BP 1, plays a crucial role in regulating the initiation of protein synthesis. It binds to the eukaryotic translation initiation factor 4E (eIF4E), thereby inhibiting the assembly of the translation initiation complex. 4E-BP1, also known as PHAS-I or PHASI, is a phosphorylated heat- and acid-stable protein that is regulated by insulin. Its name, Eif4ebp1, signifies its association with eukaryotic translation initiation factor 4E and its role as a binding protein. Understanding the function and regulation of 4E-BP1 is essential for investigating protein synthesis and its control mechanisms.
Source	Rabbit
Specificity	Total EIF4EBP1 levels can be detected by the EIF4EBP1 Antibody, which is capable of recognizing endogenous levels of the protein.
Species Reactivity	Human;Mouse;Rat
Antigen	EIF4EBP1
Application	The recommended dilution for Western blotting ranges from 1:500 to 1:2000, while for immunohistochemistry it is 1:50 to 1:200. For immunofluorescence and immunocytochemistry, the suggested dilution is 1:100 to 1:500. When conducting an enzyme-linked immunosorbent assay using a peptide, it is advised to dilute the sample from 1:20000 to 1:40000. To ensure accurate results, it is crucial to follow the recommended dilution guidelines for each application.
Immunogen	A synthesized peptide derived from human EIF4EBP1.
MW	12kDa
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography with SulfoLink™ Coupling Resin was utilized to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.
Posttranslational modification	Phosphorylated on serine and threonine residues in response to insulin, EGF and PDGF. Phosphorylation at Thr-37, Thr-46, Ser-65 and Thr-70, corresponding to the hyperphosphorylated form, is regulated by mTORC1 and abolishes binding to EIF4E.Ubiquitinated: when eIF4E levels are low, hypophosphorylated form is ubiquitinated by the BCR(KLHL25) complex, leading to its degradation and serving as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low. Not ubiquitinated when hyperphosphorylated (at Thr-37, Thr-46, Ser-65 and Thr-70) or associated with eIF4E.
Celluar localization	Cytosol;Nucleus;
UniPort	Q13541