Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-EHMT2 Polyclonal Antibody#abs135899

Please note that the price provided is only for your reference. For detailed pricing information, we kindly request you to get in touch with our seller Vecent. We encourage you to reach out to Vecent for accurate pricing details. Western blot analysis of extracts of various cell lines, using...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs135899-100ug	1-2 Weeks	301.0
abs135899-50ug	1-2 Weeks	201.0

Overview
catalog	abs135899
Description	EHMT2, also known as G9a, belongs to a family of histone lysine methyltransferases that share a catalytic SET domain. This domain was first identified in Drosophila proteins like Enhancer of zeste and Su[var]3-9, and in Trithorax proteins. In vitro experiments have demonstrated that recombinant G9a can mono-, di-, and tri-methylate histone H3 on Lys9 and Lys27. However, in vivo, G9a and GLP (a related histone methyltransferase) form a complex that functions as the major euchromatic histone H3 Lys9 mono- and di-methyltransferases, thereby creating transcriptionally repressive marks that facilitate gene silencing. G9a undergoes self-methylation on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex. The complex also contains the zinc finger protein Wiz, which is necessary for G9a/GLP hetero-dimerization and complex stability. Wiz is equipped with two CtBP co-repressor binding sites, which mediate the association of the G9a/GLP complex with the CtBP co-repressor complex. Furthermore, G9a and GLP are vital components of large transcriptional co-repressor complexes that involve E2F6 and CDP/cut. G9a interacts with DNMT1, and both proteins are essential for DNA and histone H3 (Lys9) methylation at replication foci, indicating a functional link between histone H3 Lys9 and CpG methylation during DNA replication. Furthermore, G9a activity is critical for meiotic prophase progression, as the germ line G9a-deficient mutant mice reveal a significant loss of mature gametes. Interestingly, G9a contributes to increased global levels of di-methyl histone H3 (Lys9) during hypoxic stress, and its increased expression is associated with hepatocellular carcinoma.
Other names	G9a protein, also known as EHMT2 or euchromatic histone lysine N methyltransferase 2, plays a crucial role in epigenetic regulation. This ankyrin repeat-containing protein, encoded by the C6orf30 gene, is involved in the methylation of histone H3 at lysine 9 (H3K9). It is a member of the KMT1C family and is responsible for catalyzing the transfer of methyl groups to specific sites on histone proteins. The G9a histone methyltransferase, also called H3-K9-HMTase 3, is associated with euchromatin and contributes to gene expression regulation. Its function is particularly important in maintaining the stability and integrity of the genome. Through its enzymatic activity, G9a is involved in various cellular processes, including transcriptional repression, X chromosome inactivation, and genomic imprinting. Additionally, G9a has been associated with several diseases and conditions. It is implicated in cancer progression, as aberrant G9a activity can lead to abnormal gene silencing or activation. Moreover, G9a dysregulation has been linked to neurodevelopmental disorders and psychiatric diseases. In conclusion, G9a is a crucial player in epigenetic regulation, specifically in histone methylation. Its role in gene expression and genome stability highlights its significance in various biological processes and pathological conditions.
Source	Rabbit
Specificity	EHMT2 Antibody detects endogenous levels of total EHMT2.
Species Reactivity	Human;Mouse;Rat
Antigen	EHMT2
Application	WB 1:500-1:2000, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human EHMT2.
MW	132kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLinkâ„¢ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 Â°C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 Â°C.Stable for 12 months from date of receipt.
Target
Background	Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
Tissue specificity	Expressed in all tissues examined, with high levels in fetal liver, thymus, lymph node, spleen and peripheral blood leukocytes and lower level in bone marrow.
Posttranslational modification	Methylated at Lys-185; automethylated.
Celluar localization	Nucleus;
UniPort	Q96KQ7