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Rabbit Anti-V-ATPase H Polyclonal Antibody#abs140581

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Description

Catalog-specification	Delivery time	USD price
abs140581-100ug	1-2 Weeks	301.0
abs140581-50ug	1-2 Weeks	201.0

Overview
catalog	abs140581
Other names	The ATP6V1H gene encodes for the ATPase, H+ transporting, lysosomal 50/57 kDa, V1 subunit H, which plays a crucial role in the proton transport process in lysosomes. Some of the alternative names for this gene include CGI11, NBP1, and VMA13. Additionally, the protein VMA13 homolog is one of the derivatives of ATP6V1H. The gene is located on chromosome 17 in humans, and its expression is ubiquitous across different tissues. The V-ATPase subunit H is essential for the functioning of the vacuolar proton pump. It forms part of the V1 sector of the V-ATPase enzyme complex, along with other subunits, which is responsible for ATP hydrolysis and proton translocation within the lysosome. Mutations in the ATP6V1H gene have been associated with different diseases, including osteopetrosis and distal renal tubular acidosis. SFDalpha and SFDbeta are two alternatively spliced variants of the ATP6V1H gene that were initially identified in rat kidneys. They have since been shown to contribute to the proton pumping activity of the V-ATPase complex. The Nef-binding protein 1 (NBP1) is another protein that interacts with ATP6V1H. Overall, the ATP6V1H gene and its derivatives are crucial for lysosomal acidification and several other cellular processes that rely on pH regulation. Understanding the function and regulation of these proteins will provide insights into the pathogenesis of various diseases related to lysosomal dysfunction and acidification.
Source	Rabbit
Specificity	The V-ATPase H Antibody is capable of recognizing total V-ATPase H levels present in the body. This means that it can detect endogenous levels of the protein with precision. To reiterate, the antibody is highly effective in identifying V-ATPase H, making it an invaluable tool for research purposes.
Species Reactivity	Human;Mouse
Predictive reaction species	Zebrafish;Chicken;Rabbit;Xenopus;Dog;Horse;Bovine;Sheep;Pig
Antigen	V-ATPase H
Application	The dilution ratios for WB, IHC, and ELISA (peptide) are as follows: WB at 1:1000, IHC at 1:200, and ELISA (peptide) at a range of 1:20000-1:40000. It is important to note that the information is presented in the same order as the original text, but the wording and structure are different.
Immunogen	A synthetic peptide has been developed from the human V-ATPase H. This peptide is closely related to the original protein and has been synthesized using advanced techniques. The V-ATPase H is an important component of the proton pump machinery in the human body. It plays a crucial role in maintaining the pH balance and acidification of various cellular compartments. The synthesized peptide is expected to have similar functions and properties as the original protein, making it a valuable tool for future research in the field of biochemistry and pharmacology. Overall, the development of this peptide represents a significant step towards understanding the complex mechanisms underlying the regulation of proton transport in cells.
MW	55kDa
Properties
Concentration	1mg/ml
purification	The process of purifying the antiserum involved utilizing SulfoLink™ Coupling Resin in peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	Please make sure to store it at a temperature of -20 °C for a duration of one year. Remember to avoid subjecting it to repeated freeze/thaw cycles.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Subunit of the peripheral V1 complex of vacuolar ATPase. Subunit H activates the ATPase activity of the enzyme and couples ATPase activity to proton flow. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system (By similarity). Involved in the endocytosis mediated by clathrin-coated pits, required for the formation of endosomes.
Tissue specificity	Widely expressed.
Celluar localization	Cytosol;Extracellular region or secreted;Lysosome;Plasma Membrane;
UniPort	Q9UI12